bs-12397R-RBITC

DATASHEET

Host: Rabbit

Target Protein: MAML2

Immunogen Range: 121-220/1156

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84441

Source: KLH conjugated synthetic peptide derived from human MAML2

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

Notch receptors are involved in cell-fate determination in organisms as diverse as flies, frogs, and humans (1). The 'mastermind' gene has been identified in multiple genetic screens for modifiers of Notch mutations in Drosophila melanogaster (2). In Drosophila, loss-of-function mutations of Notch produce a 'neurogenic' phenotype in which cells destined to become epidermis switch fate and differentiate to neural cells (2). The human homolog, mastermind-like 1 (Mam1), localizes to nuclear bodies (2-4). Mam1 binds to the ankyrin repeat domain of all four mammalian Notch receptors, forms a DNA-binding complex with ICN and RBP-Jk, and amplifies Notch-induced transcription of Hes1 (2). Mam1 is an essential component of the transcriptional apparatus of Notch signaling (5). The gene which encodes Mam1 maps to human chromosome 5 (4).

Conjugation: RBITC

Excitation/ Emission: 540nm/625nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 125

Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.