bs-1240R [Primary Antibody]
human Fibrinogen Polyclonal Antibody
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Host: Rabbit

Target Protein: human Fibrinogen

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2243

Swiss Prot: P02671

Source: Fibrinogen from human plasma

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen beta (FGB) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However, subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets via an ITGB3-dependent pathway. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the immune response via both innate and T-cell mediated pathways.

Size: 100µL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 95/340


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Chen, Jiang, et al. "The effect of full/partial UV-irradiation of TiO2 films on altering the behavior of fibrinogen and platelets." Colloids and Surfaces B: Biointerfaces (2014).Read more>>
  • Chen, Jiang, et al. "Effect of the Duration of UV Irradiation on the Anticoagulant Properties of Titanium Dioxide Films." ACS Applied Materials & Interfaces(2015).Read more>>
  • Wang J et al. Recombinant Human Milk Fat Globule-Epidermal Growth Factor 8 Attenuates Microthrombosis after Subarachnoid Hemorrhage in Rats. J Stroke Cerebrovasc Dis. 2019 Dec 26:104536.Read more>>
  • Eszter . Tth. et al. Formation of a protein corona on the surface of extracellular vesicles in blood plasma. J Extracell Vesicles. 2021 Sep;10(11):e1214Read more>>
  • A.D. Roseborough. et al. Fibrinogen primes the microglial NLRP3 inflammasome and propagates pro-inflammatory signaling via extracellular vesicles: Implications for blood-brain barrier dysfunction. NEUROBIOL DIS. 2023 Feb;177:106001Read more>>
  • Ya Liu. et al. Validation for the function of protein C in mouse models. PEERJ. 2024 Apr;12:e17261Read more>>
VALIDATION IMAGES

Antigen: human liver\nPrimary:Rabbit Anti-human Fibrinogen Polyclonal Antibody, 1:500\nSecondary: Goat Anti-Rabbit IgG Antibody HRP Conjugated,(bs-0295G-HRP) at 1:10,000; \nTMB staining; Read the data in MicroplateReader at 450nm.


4% Paraformaldehyde-fixed HepG2 (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (human Fibrinogen) polyclonal Antibody, unconjugated (bs-1240R) 1:100, 90 min at 37°C; followed by BF488 conjugated Goat Anti-Rabbit IgG antibody (green, bs-60295G-BF488) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.