bs-12447R [Primary Antibody]
ADAM17 (Thr735) Polyclonal Antibody
www.biossusa.com
[email protected]
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DATASHEET

Host: Rabbit

Target Protein: ADAM17 Thr735

Modification Site: Thr735

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6868

Source: KLH conjugated synthetic phosphopeptide derived from human ADAM17 around the phosphorylation site of Thr735

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq].

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 68/91


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Cow
Sheep
Pig

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded human gastric cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with ADAM17 (Thr735) Polyclonal Antibody (bs-12477R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.