bs-12491R [Primary Antibody]
APOA1BP/AI-BP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: APOA1BP/AI-BP

Immunogen Range: 201-288/288


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 128240

Source: KLH conjugated synthetic peptide derived from human APOA1BP/AI-BP

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Apolipoproteins are protein components of plasma lipoproteins. Apolipoprotein A-I (ApoA1) promotes cholesterol efflux from tissues to the liver for excretion. ApoA1 is the major protein component of high density lipoprotein (HDL) in the plasma. It can function as a cofactor for lecithin cholesterolacyltransferase, which is responsible for the formation of most plasma cholesteryl esters. AI-BP (Apolipoprotein A-I-binding protein), also known as YjeF N-terminal domain-containing protein 1, is a 288 amino acid secreted protein that binds ApoA1, ApoA2 and HDL. Individuals with impaired renal function show an increased rate of AI-BP excretion, indicating that it is normally reabsorbed within the kidney tubules. AI-BP belongs to the YjeF N-terminal domain protein family, which includes proteins that are frequently involved in oogenesis and spermatogenesis. There are two isoforms of AI-BP that are produced as a result of alternative splicing events.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 27


Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Sheep
Pig
Rabbit
Chimpanzee

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Rat Kidney tissue lysates; Lane 2: Rat Testis tissue lysates; Lane 3: Rat Liver tissue lysates; Lane 4: Rat Heart tissue lysates; Lane 5: Human 293T cell lysates; Lane 6: Human LO2 cell lysates probed with APOA1BP/AI-BP Polyclonal Antibody, Unconjugated (bs-12491R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.