bs-12568R [Primary Antibody]
BAF53A Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: BAF53A

Immunogen Range: 101-200/429


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 86

Source: KLH conjugated synthetic peptide derived from human BAF53A

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

The SWI/SNF complex regulates gene expression via ATP-dependent chromatin remodeling. Brm (SNF2-a), Brg-1 (SNF2-b), Ini1 (integrase interactor 1, SNF5), BAF53 (ARPn beta), BAF57, BAF155 (SRG3), and BAF170 make up the functional core. BAF53 homologues from yeast to humans contain a conserved N-terminal motif, which contains residues at serine 2 and tyrosine 6, which play important roles in BAF53 activity. The BAF53 protein shuttles between the nucleus and cytoplasm. BAF53 also forms a complex with TIP49 and TIP48, which mediates c-Myc oncogenic activity.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 47


Cross Reactive Species: Mouse
Rat

Predicted Cross Reactive Species: Human
Pig
Horse
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BAF53A Polyclonal Antibody, Unconjugated (bs-12568R) at 1:200 overnight at 4°C, DAB staining.


Paraformaldehyde-fixed, paraffin embedded Mouse ovarian; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with BAF53A Polyclonal Antibody, Unconjugated (bs-12568R) at 1:200 overnight at 4°C, DAB staining.