bs-1271R [Primary Antibody]
HTRA2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: HTRA2

Immunogen Range: 355-458/458


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 27429

Swiss Prot: O43464

Source: KLH conjugated synthetic peptide derived from human HtrA2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins(also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Isoform 2 seems to be proteolytically inactive. [CATALYTIC ACTIVITY] Cleavage of non-polar aliphatic amino-acids at the P1 position, with a preference for Val, Ile and Met. At the P2 and P3 positions, Arg is selected most strongly with a secondary preference for other hydrophilic residues. [SUBUNIT] Homotrimer. Interacts with MXI2. The mature protein, but not the precursor, binds to BIRC2, BIRC3 and XIAP.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 35


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Dog
Cow
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: LOVO lysates; Lane 2: TT lysates probed with HTRA2 Polyclonal Antibody, Unconjugated (bs-1271R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.


Tissue/cell: Rat Kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: HTRA2/Omi Polyclonal Antibody, Unconjugated (bs-1271R) 1:200, overnight at 4°C, followed by incubation with conjugated secondary antibody and DAB staining


Lane 1: Mouse Cerebrum lysates; Lane 2: Rat Cerebrum lysates; Lane 3: Human Hela cell lysates; Lane 4: Human U-2OS cell lysates; Lane 5: Human HT1080 cell lysates; Lane 6: Human Jurkat cell lysates probed with HtrA2/Omi Polyclonal Antibody, Unconjugated (bs-1271R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.