bs-1273R [Primary Antibody]
E-Selectin/CD62E Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: E-Selectin/CD62E

Immunogen Range: 501-612/612


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 20339

Swiss Prot: Q00690

Source: KLH conjugated synthetic peptide derived from mouse E-Selectin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 65


Cross Reactive Species: Human
Mouse
Rat
Pig

Predicted Cross Reactive Species: Dog
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Kong, Lingxi, et al. "The anti-inflammatory effect of kaempferol on early atherosclerosis in high cholesterol fed rabbits." Lipids in Health and Disease 12.1 (2013): 115.Read more>>
  • Tu, Q., et al. "Atorvastatin protects against cerebral ischemia/reperfusion injury through anti-inflammatory and antioxidant effects." Neural Regeneration Research 9.3 (2014): 268.Read more>>
  • Naito, Chisato, et al. "Facilitatory effects of fetuin-A on atherosclerosis."Atherosclerosis (2016).Read more>>
  • Wei, Min Jun, et al. "Identification of OPN, TNC and E-selectin as potential recognition proteins in cerebral vasospasm after subarachnoid hemorrhage."Chinese Neurosurgical Journal 2.1 (2016): 1.Read more>>
  • Chou, Kang-Ju, et al. "CD44 Fucosylation on Mesenchymal Stem Cell Enhances Homing and Macrophage Polarization in Ischemic Kidney Injury." Experimental Cell Research (2016).Read more>>
  • Zhang, Shengye, et al. "Role of the plasma cascade systems in ischemia/reperfusion injury of bone." Bone (2017).Read more>>
  • Zhang, Zhuochao, et al. "Cytokine profiles in Tibetan macaques following α‐1, 3‐galactosyltransferase‐knockout pig liver xenotransplantation." Xenotransplantation (2017).Read more>>
  • Zhixiong Li et al. Shuxuening injection facilitates neurofunctional recovery via down-regulation of G-CSF-mediated granulocyte adhesion and diapedesis pathway in a subacute stroke Mousemodel. Biomed Pharmacother. 2020 Jul;127:110213.Read more>>
  • Wang X et al. Crosstalk between Akt and NF\B pathway mediates inhibitory effect of gas6 on monocytes\endothelial cells interactions stimulated by P. gingivalis\LPS. J Cell Mol Med. 2020 Jul;24(14):7979-7990.Read more>>
  • Chou K et al. Secretome of Hypoxic Endothelial Cells Stimulates Bone Marrow-Derived Mesenchymal Stem Cells to Enhance Alternative Activation of Macrophages. Int J Mol Sci. 2020 Jun 21;21(12):4409.Read more>>
  • Qian Liet al. LOX-1 Regulates P. gingivalis-Induced Monocyte Migration and Adhesion to Human Umbilical Vein Endothelial Cells. Front Cell Dev Biol. 2020 Jul 14;8:596.Read more>>
  • Na Li. et al. SELE gene as a characteristic prognostic biomarker of colorectal cancer:. J Int Med Res. 2021;49(4)Read more>>
  • Wen Xiong. et al. Mechanisms and Molecular Targets of BuShenHuoXue Formula for Osteoarthritis. Acs Omega. 2022;7(5):47034713Read more>>
  • Masahiro Terasawa. et al. Anti-Inflammatory Activity of Orally Administered Monostroma nitidum Rhamnan Sulfate against Lipopolysaccharide-Induced Damage to Mouse Organs and Vascular Endothelium. Mar Drugs. 2022 Feb;20(2):121Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-E-Selectin Polyclonal Antibody, Unconjugated(bs-1273R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: DU145 cell lysates; Lane 2: LoVo cell lysates; probed with E-Selectin/CD62E Polyclonal Antibody, Unconjugated (bs-1273R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.