bs-12766R [Primary Antibody]
VPS13D Polyclonal Antibody
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Host: Rabbit

Target Protein: VPS13D

Immunogen Range: 1651-1750/4388


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 55187

Swiss Prot: Q5THJ4

Source: KLH conjugated synthetic peptide derived from human VPS13D

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]

Size: 100uL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 492


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Jian Wang. et al. VPS13D affects epileptic seizures by regulating mitochondrial fission and autophagy in epileptic rats. GENES DIS. 2024 Mar;:101266Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VPS13D Polyclonal Antibody, Unconjugated (bs-12766R) at 1:400 overnight at 4°C, DAB staining.


U-2OS cells were stained with VPS13D Polyclonal Antibody, Unconjugated(bs-12766R) at 1:100 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) FITC conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.