bs-1278R
[Primary Antibody]
8-OHdG Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: 8-OHdG/8 Hydroxyguanosine
Clonality: Polyclonal
Isotype: IgG
Source: KLH conjugated 8-OhdG
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease.
Size: 100ul
Concentration: 1ug/ul
Applications:
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 0.283
Cross Reactive Species:
Human
Mouse
Rat
Others
(8-OHdG)
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Wang J et al. PGC-1α regulate critical period plasticity via gene× environment interaction in the developmental trajectory to schizophrenia. Biochem Biophys Res Commun. 2020 May 14;525(4):989-996.Read more>>
- Zhang, Ting-Ting, et al. "Electrochemiluminescence immunosensor for highly sensitive detection of 8-hydroxy-2′-deoxyguanosine based on carbon quantum DoT coated Au/SiO2 core-shell nanoparticles." Talanta (2014).Read more>>
- Stangenberg, Stefanie, et al. "Oxidative Stress, Mitochondrial Perturbations and Fetal Programming of Renal Disease induced by Maternal Smoking." The International Journal of Biochemistry & Cell Biology (2015).Read more>>
- Feng, Demin, et al. "Ameliorative effects of N-acetylcysteine on fluoride-induced oxidative stress and DNA damage in male rats’ testis." Mutation Research/Genetic Toxicology and Environmental Mutagenesis 792 (2015): 35-45.Read more>>
- Pan, Deng, et al. "Electrochemical immunoassay for the biomarker 8-hydroxy-2′-deoxyguanosine using a glassy carbon electrode modified with chitosan and poly (indole-5-carboxylic acid)." Microchimica Acta (2015): 1-8.Read more>>
- Tumurkhuu, Gantsetseg, et al. "Ogg1-Dependent DNA Repair Regulates NLRP3 Inflammasome and Prevents Atherosclerosis." Circulation Research(2016): CIRCRESAHA-116.Read more>>
- Yan, Jiawei, et al. "56Fe irradiation-induced cognitive deficits through oxidative stress in mice." Toxicology Research (2016).Read more>>
- Yu, Dan, et al. "In vitro the differences of inflammatory and oxidative reactions due to sulfur mustard induced acute pulmonary injury underlying intraperitoneal injection and intratracheal instillation in rats." International Immunopharmacology 47 (2017): 78-87.Read more>>
- Du, Xiaoping, et al. "Antioxidants Reduce Neurodegeneration and Accumulation of Pathologic Tau Proteins in the Auditory System after Blast Exposure." Free Radical Biology and Medicine (2017).Read more>>
- Narayanaswamy et al. Transcriptomic pathway analysis of urokinase receptor silenced breast cancer cells: a microarray study. (2017) Oncotarget. 8:101572-101590Read more>>
- Wu et al. Curcumin pretreatment and post-treatment both improve the antioxidative ability of neurons with oxygen-glucose deprivation. (2015) Neural.Regen.Res. 10:481-9Read more>>
- Li et al. Hyperglycaemia exacerbates choroidal neovascularisation in mice via the oxidative stress-induced activation of STAT3 signalling in RPE cells. (2012) PLoS.One. 7:e47600Read more>>
- Wang R et al. Leonurine alleviates LPS-induced myocarditis through suppressing the NF-кB signaling pathway. Toxicology. 2019 Apr 18;422:1-13.Read more>>
- Zhou J et al. Protective effect of Dioscorea zingiberensis ethanol extract on the disruption of blood–testes barrier in high‐fat diet/streptozotocin‐induced diabetic mice by upregulating ZO-1 and Nrf2. Andrologia. 2020 Jan 20:e13508. Read more>>
- Parma L et al. Prolonged Hyperoxygenation Treatment Improves Vein Graft Patency and Decreases Macrophage Content in Atherosclerotic Lesions in ApoE3* Leiden Mice. Cells. 2020 Feb 1;9(2). pii: E336. Read more>>
- Nanizawa E et al. Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state. Biochem Biophys Rep. 2020 Feb 12;22:100736.Read more>>
- Wang J et al. PGC-1α regulate critical period plasticity via gene× environment interaction in the developmental trajectory to schizophrenia. Biochem Biophys Res Commun. 2020 May 14;525(4):989-996.Read more>>
- Luo HM et al. Calcitonin gene\related peptide inhibits angiotensin II\induced NADPH oxidase\dependent ROS via the Src/STAT3 signalling pathway. J Cell Mol Med. 2020 Jun;24(11):6426-6437.Read more>>
- Yi Luet al. Activation of NRF2 ameliorates oxidative stress and cystogenesis in autosomal dominant polycystic kidney disease. Sci Transl Med. 2020 Jul 29;12(554):eaba3613.Read more>>
- Yukie Mizutaet al. Sodium thiosulfate prevents doxorubicin-induced DNA damage and apoptosis in cardiomyocytes in mice. Life Sci. 2020 Sep 15;257:118074.Read more>>
- Jia-Feng Changet al. Scavenging Intracellular ROS Attenuates p-Cresyl Sulfate-Triggered Osteogenesis through MAPK Signaling Pathway and NF-B Activation in Human Arterial Smooth Muscle Cells. Toxins (Basel). 2020 Jul 24;12(8):472.Read more>>
- Sun X et al. Increased MAO-A Activity Promotes Progression of Pulmonary Arterial HypertensionAm J Respir Cell Mol Biol.2020 Dec 2.Read more>>
- Gomez-Puerto MC et al. MnTBAP Reverses Pulmonary Vascular Remodeling and Improves Cardiac Function in Experimentally Induced Pulmonary Arterial Hypertension. Int J Mol Sci. 2020 Jun 10;21(11):4130.Read more>>
- Wanyue Huang. et al. Aflatoxin B1 disrupts blood-testis barrier integrity by reducing junction protein and promoting apoptosis in mice testes. Food Chem Toxicol. 2021 Feb;148:111972Read more>>
- Xiao-Fan Huang. et al. Pogostemon cablin Triggered ROS-Induced DNA Damage to Arrest Cell Cycle Progression and Induce Apoptosis on Human Hepatocellular Carcinoma In Vitro and In Vivo. Molecules. 2020 Jan;25(23):5639Read more>>
- Shotaro Hayashi. et al. Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice. Redox Biol. 2020 Oct;37:101726Read more>>
- Chaoqun Hu. et al. Urine-derived stem cells accelerate the recovery of injured mouse hepatic tissue. Am J Transl Res. 2020; 12(9): 5131C515Read more>>
- Yuan-Feng Dai. et al. Association of pepsin and DNA damage in laryngopharyngeal reflux-related vocal fold polyps. Am J Otolaryng. 2020 Nov;41:102681Read more>>
- enjamin P. Larkin. et al. Low-dose hydralazine during gestation reduces renal fibrosis in rodent offspring exposed to maternal high fat diet. Plos One. 2021 Mar;16(3):e0248854Read more>>
- Wei Ou. et al. Hypoxic acclimation improves cardiac redox homeostasis and protects heart against ischemia-reperfusion injury through upregulation of O-GlcNAcylation. Redox Biol. 2021 Jul;43:101994Read more>>
- Hongxiao Yang. et al. Hydrogen Attenuates Myocardial Injury in Rats by Regulating Oxidative Stress and NLRP3 Inflammasome Mediated Pyroptosis. Int J Med Sci. 2021; 18(14): 3318C3325Read more>>
- enjamin P. Larkin. et al. Novel Role of Gestational Hydralazine in Limiting Maternal and Dietary Obesity-Related Chronic Kidney Disease. Front Cell Dev Biol. 2021; 9: 705263Read more>>
- Ms. Nannan Zhang. et al. Characterization of Urine Derived Stem Cells from Patients with End-Stage Liver Diseases and Application to Induced Acute and Chronic Liver Injury of Nude Mice Model. 2021 Sep 22Read more>>
- Zhihui Tian. et al. The activated ATM/p53 pathway promotes autophagy in response to oxidative stress-mediated DNA damage induced by Microcystin-LR in male germ cells. Ecotox Environ Safe. 2021 Dec;227:112919Read more>>
- Sydney L. Lane. et al. Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice. Int J Mol Sci. 2021 Jan;22(23):13019Read more>>
- Ma Yu-Bo. et al. Oxidative Stress Disrupted Prepubertal Rat Testicular Development after Xenotransplantation. Oxid Med Cell Longev. 2021;2021:169999Read more>>
- Tao Huang. et al. Cordycepin, a major bioactive component of Cordyceps militaris, ameliorates diabetes-induced testicular damage through the Sirt1/Foxo3a pathway. 2021 Nov 22Read more>>
- Yuan Chen. et al. Intrinsic Radical Species Scavenging Activities of Tea Polyphenols Nanoparticles Block Pyroptosis in Endotoxin-Induced Sepsis. Acs Nano. 2022;XXXX(XXX):XXX-XXXRead more>>
- Tao Gui. et al. Superoxide dismutase-loaded porous polymersomes as highly efficient antioxidant nanoparticles targeting synovium for osteoarthritis therapy. Biomaterials. 2022 Apr;283:121437Read more>>
- Zhao Mingyue. et al. NRF2 pathway activation attenuates ageing-related renal phenotypes due to _-klotho deficiency. J Biochem. 2022 FebRead more>>
- Eroglu, Binnur. et al. Dusp26 phosphatase regulates mitochondrial respiration and oxidative stress and protects neuronal cell death. Cell Mol Life Sci. 2022 Apr;79(4):1-28Read more>>
- Ryota Murata. et al. Long-Acting Thioredoxin Ameliorates Doxorubicin-Induced Cardiomyopathy via Its Anti-Oxidative and Anti-Inflammatory Action. Pharmaceutics. 2022 Mar;14(3):562Read more>>
- Hongxiao Yang. et al. Hydrogen Attenuates Thyroid Hormone-Induced Cardiac Hypertrophy in Rats by regulating angiotensin II type 1 receptor and NADPH oxidase 2 mediated oxidative stress. Eur J Pharmacol. 2022 May;922:174917Read more>>
- Enoch Odame Anto. et al. Placental lesions and differential expression of pro-and anti-angiogenic growth mediators and oxidative DNA damage marker in placentae of Ghanaian suboptimal and optimal health status pregnant women who later developed preeclampsia. Plos One. 2022 Mar;17(3):e0265717Read more>>
- Xin, Liangjing. et al. Four-Octyl itaconate ameliorates periodontal destruction via Nrf2-dependent antioxidant system. INT J ORAL SCI. 2022 May;14(1):1-12Read more>>
- Shou-En Wu. et al. Benzo[a]pyrene exposure in muscle triggers sarcopenia through aryl hydrocarbon receptor-mediated reactive oxygen species production. ECOTOX ENVIRON SAFE. 2022 Jul;239:113599Read more>>
- Shen, Hao. et al. Hepatocyte-derived VEGFA accelerates the progression of non-alcoholic fatty liver disease to hepatocellular carcinoma via activating hepatic stellate cells. ACTA PHARMACOL SIN. 2022 May;:1-12Read more>>
- Anil Ahsan. et al. Potential Chemotherapeutic Effect of Selenium for Improved Canceration of Esophageal Cancer. INT J MOL SCI. 2022 Jan;23(10):5509Read more>>
- Jia-Feng Chang. et al. Translational Medicine in Uremic Vascular Calcification: Scavenging ROS Attenuates p-Cresyl Sulfate-Activated Caspase-1, NLRP3 Inflammasome and Eicosanoid Inflammation in Human Arterial Smooth Muscle Cells. LIFE-BASEL. 2022 May;12(5):769Read more>>
- Deng Disi. et al. Protective Effect of XinJiaCongRongTuSiZiWan on the Reproductive Toxicity of Female Rats Induced by Triptolide. EVID-BASED COMPL ALT. 2022;2022:3642349Read more>>
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded Human breast cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with 8-OHdG Polyclonal Antibody, Unconjugated (bs-1278R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell: rat brain tissue(left panel was injury,Right panel was normal); 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-8-OHdG Polyclonal Antibody, Unconjugated (bs-1278R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining