bs-1281R [Primary Antibody]
XIAP Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: XIAP/BIRC4

Immunogen Range: 201-330/496


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11798

Swiss Prot: Q60989

Source: KLH conjugated synthetic peptide derived from mouse XIAP

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage: Store at -20°C for 12 months..

Background:

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 55


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Tan J et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020 Jun;126:110096.Read more>>
  • Chen, Xin Mei, et al. "Ampelopsin induces apoptosis by regulating multiple c‑Myc S‑phase kinase‑associated protein 2 F‑box and WD repeat‑containing protein 7 histone deacetylase 2 pathways in human lung adenocarcinoma cells." Molecular Medicine Reports.Read more>>
  • Sun, Yiming, et al. "Mechanisms underlying 3-bromopyruvate-induced cell death in colon cancer." Journal of Bioenergetics and Biomembranes (2015): 1-11.Read more>>
  • Jin, Gongsheng, et al. "Smac mimetic‑induced caspase‑independent necroptosis requires RIP1 in breast cancer." Molecular Medicine Reports (2015).Read more>>
  • Sun, Y‐L., et al. "Relationship between apoptosis and proliferation in granulosa and theca cells of cystic follicles in sows." Reproduction in domestic animals 47.4 (2012): 601-608.Read more>>
  • Chen J et al. SM‑164 enhances the antitumor activity of adriamycin in human U2‑OS cells via downregulation of X‑linked inhibitor of apoptosis protein. Mol Med Rep. 2019 Jun;19(6):5079-5086.Read more>>
  • Tan J et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020 Jun;126:110096.Read more>>
  • Lei Tian. et al. Acute ozone exposure can cause cardiotoxicity: Mitochondria play an important role in mediating myocardial apoptosis. Chemosphere. 2021 Apr;268:128838Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human endometrial tissue labeled with Anti-XIAP/BIRC4 Polyclonal Antibody, Unconjugated (bs-1281R) followed by conjugation to the secondary antibody and DAB staining


Image provided by One World Lab validation program. A549 cells probed with Rabbit Anti-XIAP Polyclonal Antibody (bs-1281R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.


Image provided by One World Lab validation program. MCF-7 cells probed with Rabbit Anti-XIAP Polyclonal Antibody (bs-1281R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.\n