Host: Rabbit
Target Protein: BAK
Immunogen Range: 21-120/209
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 12018
Swiss Prot: O08734
Source: KLH conjugated synthetic peptide derived from mouse BAK
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 23
Cross Reactive Species:
Human
Mouse
Rat
Chicken
For research use only. Not intended for diagnostic or therapeutic use.
- Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245. Read more>>
- Huang, Chuen-Lin, et al. "Paraquat Induces Cell Death Through Impairing Mitochondrial Membrane Permeability." Molecular Neurobiology (2015): 1-20.Read more>>
- Peng et al. Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells. (2018) Oncol.Lett. 16:785-792Read more>>
- Yang F et al. Copper induces oxidative stress and apoptosis through mitochondria-mediated pathway in chicken hepatocytes.(2019)Toxicol In Vitro. Feb;54:310-316. Read more>>
- Liu T et al. MicroRNA-193b-3p regulates hepatocyte apoptosis in selenium-deficient broilers by targeting MAML1.J Inorg Biochem. 2018 Sep;186:235-245. Read more>>
- Lu Ma. et al. Oxaliplatin promotes siMAD2L2?induced apoptosis in colon cancer cells. Mol Med Rep. 2021 Sep;24(3):1-9Read more>>
- Hu, Zhuoying. et al. Effects of Long-Term Exposure to Copper on Mitochondria-Mediated Apoptosis in Pig Liver. BIOL TRACE ELEM RES. 2022 Jun;:1-14Read more>>
- Li, Guang. et al. Synergetic delivery of artesunate and isosorbide 5-mononitrate with reduction-sensitive polymer nanoparticles for ovarian cancer chemotherapy. J NANOBIOTECHNOL. 2022 Dec;20(1):1-13Read more>>
- Qiyu Gan. et al. Prognostic Value and Immune Infiltration of HPV-Related Genes in the Immune Microenvironment of Cervical Squamous Cell Carcinoma and Endocervical Adenocarcinoma. CANCERS. 2023 Jan;15(5):1419Read more>>
- Qingyu Ding. et al. N-acetylcysteine alleviates oxidative stress and apoptosis and prevents skeletal muscle atrophy in type 1 diabetes mellitus through the NRF2/HO-1 pathway. LIFE SCI. 2023 Sep;329:121975Read more>>
Lane 1: Mouse lung lysate probed with Rabbit Anti-BAK Polyclonal Antibody, Unconjugated (bs-1284R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90 min at RT.
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Bak Polyclonal Antibody, Unconjugated (bs-1284R) followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded human lung carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-BAK Polyclonal Antibody, Unconjugated (bs-1284R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.
THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CXCL2 Polyclonal Antibody(bs-1284R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).