bs-1287R [Primary Antibody]
NFKBIA/IKB alpha Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFKBIA/IKB alpha

Immunogen Range: 1-120/314


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 18035

Swiss Prot: Q9Z1E3

Source: KLH conjugated synthetic peptide derived from mouse IKB alpha

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 36


Cross Reactive Species: Human
Mouse
Rat
Bovine
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Wang X et al. trans-Cinnamaldehyde Reverses Depressive-Like Behaviors in Chronic Unpredictable Mild Stress Rats by Inhibiting NF-κB/NLRP3 Inflammasome Pathway. Evid Based Complement Alternat Med. 2020 Feb 28;2020:4572185.Read more>>
  • Jiang, Wang-Lin, et al. "Neuroprotective efficacy and therapeutic window of Forsythoside B: in a rat model of cerebral ischemia and reperfusion injury." European journal of pharmacology 640.1 (2010): 75-81.Read more>>
  • Yan, Xuerui, et al. "Cyanidin-3-O-glucoside Attenuates Acute Lung Injury in Sepsis Rats." Journal of Surgical Research (2015).Read more>>
  • Yan, Xuerui, et al. "Cyanidin-3-O-glucoside Induces Apoptosis and Inhibits Migration of Tumor Necrosis Factor-α-Treated Rat Aortic Smooth Muscle Cells." Cardiovascular Toxicology (2015): 1-9.Read more>>
  • Ma, Juan, et al. "A Crucial Role of Lateral Size for Graphene Oxide in Activating Macrophages and Stimulating Pro-inflammatory Responses in Cells and Animals." ACS Nano (2015).Read more>>
  • Zhao, Hongyu, et al. "Betulin attenuates lung and liver injuries in sepsis."International Immunopharmacology 30 (2016): 50-56.Read more>>
  • Zhao, Hongyu, et al. "Betulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathway."Life Sciences (2016).Read more>>
  • Li, Yuanchao, et al. "Valproate ameliorates nitroglycerin-induced migraine in trigeminal nucleus caudalis in rats through inhibition of NF-кB." The Journal of Headache and Pain 17.1 (2016): 1-9.Read more>>
  • Gao, Wenyan, et al. "Indirubin ameliorates dextran sulfate sodium-induced ulcerative colitis in mice through the inhibition of inflammation and the induction of Foxp3-expressing regulatory T cells." Acta Histochemica (2016).Read more>>
  • Yu, Haijie, et al. "Gypenoside Protects Cardiomyocytes against Ischemia-Reperfusion Injury via the Inhibition of Mitogen-Activated Protein Kinase Mediated Nuclear Factor Kappa B Pathway In Vitro and In Vivo." Frontiers in Pharmacology 7 (2016).Read more>>
  • Sun, Guodong, et al. "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." Experimental Animals: 16-0102.Read more>>
  • Ge, Lingqing, et al. "Design and discovery of novel thiazole derivatives as potential MMP inhibitors to protect against acute lung injury in sepsis rats via attenuation of inflammation and apoptotic oxidative stress." RSC Advances7.52 (2017): 32909-32922.Read more>>
  • Liu, Xinwei, et al. "Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats." Molecular Medicine Reports 16.1 (2017): 192-200.Read more>>
  • Zhao, Dongmei, et al. "Unfractionated heparin protects the protein C system against lipopolysaccharide‑induced damage in vivo and in vitro." Experimental and Therapeutic Medicine.Read more>>
  • Han et al. Zisheng Shenqi decoction ameliorates monosodium urate crystal-induced gouty arthritis in rats through anti-inflammatory and anti-oxidative effects. (2016) Mol.Med.Re. 14:2589-97Read more>>
  • Zhang et al. Hyperbaric Oxygen and Ginkgo Biloba Extract Ameliorate Cognitive and Memory Impairment via Nuclear Factor Kappa-B Pathway in Rat Model of Alzheimer's Disease. (2015) Chin.Med.J.(Engl. 128:3088-93Read more>>
  • Tang et al. 14-3-3β Promotes Migration and Invasion of Human Hepatocellular Carcinoma Cells by Modulating Expression of MMP2 and MMP9 through PI3K/Akt/NF-κB Pathway. (2016) PLoS.On. 11:e0146070Read more>>
  • Chen et al. Naringin Alleviates Diabetic Kidney Disease through Inhibiting Oxidative Stress and Inflammatory Reaction. (2015) PLoS.On. 10:e0143868Read more>>
  • Zhu et al. S-Allylmercaptocysteine Attenuates  Cisplatin-Induced Nephrotoxicity through  Suppression of Apoptosis, Oxidative Stress, and  Inflammation. (2017) Nutrient. 9 Read more>>
  • Wang et al. Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-κB Signaling. (2018) Med.Sci.Monit. 24:1282-1294Read more>>
  • Zhao et al. Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damagein vivoandin vitro. (2017) Exp.Ther.Med. 14:5515-5522Read more>>
  • Zhang et al. Apelin-13 protects against myocardial infarction-induced myocardial fibrosis. (2016) Mol.Med.Rep. 13:5262-8Read more>>
  • Gong et al. Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4)-Mediated Signaling. (2018) Med.Sci.Monit. 24:3514-3523Read more>>
  • Liu,et al.Human umbilical cord mesenchymal stem cell conditioned medium attenuates renal fibrosis by reducing inflammation and epithelial-to-mesenchymal transition via the TLR4/NF-κB signaling pathway in vivo and in vitro.(2018) Stem Cell Research & Therapy. 9:7.Read more>>
  • Cross,et al.International survey on high- and low-dose synacthen test and assessment of accuracy in preparing low-dose synacthen.(2018) Clinical Endocrinology. 88:744-751.Read more>>
  • Ma M et al. LncRNA XIST mediates bovine mammary epithelial cell inflammatory response via NF‐κB/NLRP3 inflammasome pathway.(2018) Cell Prolif.Read more>>
  • Li Y et al. Silencing of LncRNA Steroid Receptor RNA Activator attenuates polycystic ovary syndrome in Mice. (2018)Biochimie; Nov 1;157:48-56.Read more>>
  • Xin H et al. miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα. BMC Cancer. 2019 Jan 8;19(1):30. Read more>>
  • Han X et al. SUMOylation and deacetylation affect NF-κB p65 activity induced by high glucose in human lens epithelial cells. Int J Ophthalmol. 2019 Sep 18;12(9):1371-1379. Read more>>
  • Zhou J et al. Paeonol antagonizes oncogenesis of osteosarcoma by inhibiting the function of TLR4/MAPK/NF-κB pathway. Acta Histochem. 2019 Oct 3:151455. Read more>>
  • Fang D et al. Overexpression of Biglycan is Associated with Resistance to Rapamycin in Human WERI-Rb-1 Retinoblastoma Cells by Inducing the Activation of the Phosphatidylinositol 3-Kinases (PI3K)/Akt/Nuclear Factor kappa B (NF-κB) Signaling Pathway. Med Sci Monit. 2019 Sep 4;25:6639-6648. Read more>>
  • Fang D et al. Overexpression of Biglycan is Associated with Resistance to Rapamycin in Human WERI-Rb-1 Retinoblastoma Cells by Inducing the Activation of the Phosphatidylinositol 3-Kinases (PI3K)/Akt/Nuclear Factor kappa B (NF-κB) Signaling Pathway. Med Sci Monit. 2019 Sep 4;25:6639-6648. Read more>>
  • Wu ZY et al. Baicalin Attenuates Mycoplasma gallisepticum-Induced Inflammation via Inhibition of the TLR2-NF-κB Pathway in Chicken and DF-1 Cells. Infection and Drug Resistance 2019:12 3911–3923.Read more>>
  • Peng LY et al. Protective Effect of Piceatannol Against Acute Lung Injury Through Protecting the Integrity of Air-Blood Barrier and Modulating the TLR4/NF-κB Signaling Pathway Activation. Front Pharmacol. 2020 Jan 22;10:1613.Read more>>
  • Zou P et al. Protective effect of isoliquiritigenin against cerebral injury in septic mice via attenuation of NF-κB.Inflammopharmacology. 2018 Jun 26.Read more>>
  • Wang X et al. trans-Cinnamaldehyde Reverses Depressive-Like Behaviors in Chronic Unpredictable Mild Stress Rats by Inhibiting NF-κB/NLRP3 Inflammasome Pathway. Evid Based Complement Alternat Med. 2020 Feb 28;2020:4572185.Read more>>
  • Peng L et al. Madecassoside Protects Against LPS-Induced Acute Lung Injury via Inhibiting TLR4/NF-B Activation and Blood-Air Barrier Permeability. Front Pharmacol. 2020 Jun 5;11:807.Read more>>
  • Xiumei Chenet al. Hydroxysafflor Yellow A of Carthamus Tinctorius L., Represses the Malignant Development of Esophageal Cancer Cells via Regulating NF-B Signaling Pathway. Cell Biochem Biophys. 2020 Dec;78(4):511-520.Read more>>
  • Peng S et al. Poly(ADP?ribose) polymerase?1 inhibitor ameliorates dextran sulfate sodium?induced colitis in mice by regulating the balance of Th17/Treg cells and inhibiting the NF?B signaling pathwayExp Ther Med.2021 Feb;21(2):134.Read more>>
  • Xiaoyan Xia. et al. Ameliorative effect of white tea from 50-year-old tree of Camellia sinensis L. (Theaceae) on kidney damage in diabetic mice via SIRT1/AMPK pathway. J Ethnopharmacol. 2021 May;272:113919Read more>>
  • Xianmei Liu. et al. Tropomodulin1 Expression Increases Upon Maturation in Dendritic Cells and Promotes Their Maturation and Immune Functions. Front Immunol. 2020; 11: 587441Read more>>
  • Ryo Sasaki. et al. Trans-portal hepatic infusion of cultured bone marrow-derived mesenchymal stem cells in a steatohepatitis murine model. 2020 Nov 01Read more>>
  • Ge Guan. et al. The promotion of tetrabromobisphenol A exposure on Ishikawa cells proliferation and pivotal role of ubiquitin-mediated IB degradation. Ecotox Environ Safe. 2021 Jan;207:111254Read more>>
  • Feng Gao. et al. Aucubin ameliorates the LPS\induced inflammatory response in bovine endometrial epithelial cells by inhibiting NF\B and activating the Keap1/Nrf2 signalling pathway. 2021 Apr 27Read more>>
  • Qihe Tang. et al. Bergenin Monohydrate Attenuates Inflammatory Response via MAPK and NF-B Pathways Against Klebsiella pneumonia Infection. Front Pharmacol. 2021; 12: 651664Read more>>
  • Yaping Wang. et al. Blockade of GITRL/GITR signaling pathway attenuates house dust mite-induced allergic asthma in mice through inhibition of MAPKs and NF-B signaling. Mol Immunol. 2021 Sep;137:238Read more>>
  • Qingyu Meng. et al. Morin hydrate inhibits atherosclerosis and LPS-induced endothelial cells inflammatory responses by modulating the NFB signaling-mediated autophagy. Int Immunopharmacol. 2021 Nov;100:108096Read more>>
  • Shiyan Chen. et al. Lactobacillus johnsonii L531 Alleviates the Damage Caused by Salmonella Typhimurium via Inhibiting TLR4, NF-B, and NLRP3 Inflammasome Signaling Pathways. Microorganisms. 2021 Sep;9(9):1983Read more>>
  • Wang Xueqing. et al. Comparison of the Protective Effect of Different Mild Therapeutic Hypothermia Temperatures on Intestinal Injury After Cardiopulmonary Resuscitation in Rats. Shock. 2021 Sep;56(3):450-46Read more>>
  • Yongli Zhang. et al. Licorice extract ameliorates hyperglycemia through reshaping gut microbiota structure and inhibiting TLR4/NF-_B signaling pathway in type 2 diabetic mice. Food Res Int. 2022 Mar;153:110945Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat kidney labeled with Rabbit Anti-NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Lane 1: Mouse Spleen lysates probed with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 60 min at 37˚C.


Hela lysates probed with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


HepG2 lysates probed with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Thymus lysates probed with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Mouse Lymph node lysates; Lane 2: Mouse Thymus lysates probed with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:200 overnight at 4°C, DAB staining.


Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃ and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained withNFKBIA/IKB alpha Polyclonal Antibody(bs-1287R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).


Lane 1: Mouse Stomach lysates; Lane 2: Rat Stomach lysates probed with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Human HepG2 cell lysates; Lane 2: Human Hela cell lysates; Lane 3: Human Siha cell lysates; Lane 4: Human U251 cell lysates; Lane 5: Mouse Spleen lysates; Lane 6: Mouse Thymus lysates; Lane 7: Rat Thymus lysates; Lane 8: Mouse Lymph node lysates; Lane 9: Rat Lymph node lysates probed with NFKBIA/IKB alpha Polyclonal Antibody, Unconjugated (bs-1287R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Tissue/cell:Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Antibody incubation with (IKB alpha) polyclonal Antibody, Unconjugated (bs-1287R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.