bs-1292R [Primary Antibody]
Aromatase Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Aromatase

Immunogen Range: 41-140/503


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1588

Swiss Prot: P11511

Source: KLH conjugated synthetic peptide derived from human Aromatase

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis, three successive hydroxylations of the A ring of androgens. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. The gene expresses two transcript variants. Belongs to the cytochrome P450 family.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 58


Cross Reactive Species: Human
Mouse
Rat
Bovine
Others

Predicted Cross Reactive Species: Dog
Cow
Sheep
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Lan, Chen-Wei, et al. "Differentiation of Human Embryonic Stem Cells into Functional Ovarian Granulosa-like Cells."?Journal of Clinical Endocrinology & Metabolism?(2013).Read more>>
  • Gan, X., et al. "Establishment of an in vitro culture model of theca cells from hierarchical follicles in ducks." Bioscience reports (2017).Read more>>
  • Meseke M et al. Distal dendritic enrichment of HCN1 channels in hippocampal CA1 is promoted by estrogen, but does not require Reelin.(2018) eNeuro, 5 (5) ENEURO.0258-18.2018;Read more>>
  • Meseke et al. Distal Dendritic Enrichment of HCN1 Channels in Hippocampal CA1 Is Promoted by Estrogen, but Does Not Require Reelin. (2018) Eneuro.Oct 15;5(5). Read more>>
  • Song L et al. DHT and E2 synthesis-related proteins and receptors expression in male yak skin during different hair follicle stages. Gen Comp Endocrinol. 2019 Aug 12:113245. Read more>>
  • Zhong Yuyi. et al. MIR143 Inhibits Steroidogenesis and Induces Apoptosis Repressed by H3K27me3 in Granulosa Cells. Front Cell Dev Biol. 2020 Oct;8:1159Read more>>
  • Jisheng Wang. et al. In vitro and in vivo investigation of the therapeutic mechanism of Lycium Chinense and Cuscutae Semen on oligoasthenozoospermia. 2021 Mar 05Read more>>
  • Yan Deng. et al. Molecular characterization, expression profile and transcriptional regulation of the CYP19 gene in goose ovarian follicles. Gene. 2022 Jan;806:145928Read more>>
  • Yuan-Xue Jing. et al. N6-methyladenosine demethylase FTO related to hyperandrogenism in PCOS via AKT pathway. GYNECOL ENDOCRINOL. 2023;39(1):Article: 2276167Read more>>
VALIDATION IMAGES

RSC96 cells probed with Aromatase Polyclonal Antibody, Unconjugated (bs-1292R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti CYP19/CYP19A1 Polyclonal Antibody, Unconjugated (bs-1292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti CYP19/CYP19A1 Polyclonal Antibody, Unconjugated (bs-1292R) at 1:200 followed by conjugation to the secondary antibody and DAB staining