bs-13021R [Primary Antibody]
DNA polymerase mu Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: DNA polymerase mu

Immunogen Range: 261-360/494


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 27434

Source: KLH conjugated synthetic peptide derived from human DNA polymerase mu

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

DNA polymerase mu shares a number of characteristics with DNA polymerase Beta as well as with terminal deoxynucleotideyltransferase. Pol mu purportedly plays a role in microhomology mediated joining and the repair of double-stranded breaks. However, unlike other DNA polymerases, which show substrate specificity for deoxynucleotides, DNA Pol mu incorporates both deoxynucleotides and ribonucleotides in a template- directed manner. This unusual capability implies a novel role for this polymerase in DNA repair.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 55


Cross Reactive Species: Human

Predicted Cross Reactive Species: Mouse
Rat
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Deng et al. DNA Damage Signaling Is Required for Replication of Human Bocavirus 1 DNA in Dividing HEK293 Cells. (2017) J.Virol.Dec 16;91(1). Read more>>
VALIDATION IMAGES

HepG2 cell lysates probed with DNA polymerase mu Polyclonal Antibody, Unconjugated (bs-13021R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Raji cell lysates probed with DNA polymerase mu Polyclonal Antibody, Unconjugated (bs-13021R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.