Host: Rabbit
Target Protein: eEF1A1+2
Specificity: The antibody may recognize both eEF1A1 and eEF1A2 due to high sequence similarity
Immunogen Range: 1-100/463
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 1915, 1917
Source: KLH conjugated synthetic peptide derived from human eEF1A2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)
Predicted Molecular Weight: 50
Cross Reactive Species: Mouse
Predicted Cross Reactive Species:
Human
Rat
Zebrafish
For research use only. Not intended for diagnostic or therapeutic use.
Mouse heart and brain lysates probed with Rabbit Anti-eEF1A1+2 Polyclonal Antibody, Unconjugated (bs-13054R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with eEF1A1+2 Polyclonal Antibody, Unconjugated (bs-13054R) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.