bs-1305R [Primary Antibody]
CCR7/CD197 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CCR7/CD197

Immunogen Range: 25-59/379


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 12775

Swiss Prot: P47774

Source: KLH conjugated synthetic peptide derived from mouse CCR7

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

CCR7 is a member of the G protein coupled receptor family (subfamily : chemokine). This receptor was identified as a gene induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. It is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:500-2000)
ELISA(1:5000-10000)
FCM(1:100)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100)
ICC(1:100)

Predicted Molecular Weight: 42


Cross Reactive Species: Human
Mouse
Rat
Sheep

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • en Menachem-Zidon O et al. Age-associated differences in macrophage response in a vaginal wound healing rat model. Int Urogynecol J. 2020 Feb 27.Read more>>
  • Blomgran, Parmis, et al. "A possible link between loading, inflammation and healing: Immune cell populations during tendon healing in the rat." Scientific Reports 6 (2016): 29824.Read more>>
  • "Li, Xuelu, et al. ""High Expression of CCR7 Predicts Lymph Node Metastasis and Good Prognosis in Triple Negative Breast Cancer."" Cellular Physiology and Biochemistry 43.2 (2017): 531-539. "Read more>>
  • Dijk et al. Systematic Evaluation of the Cellular Innate Immune Response During the Process of Human Atherosclerosis. (2016) J.Am.Heart.Assoc. 5 Read more>>
  • Expression of Pref-1 and Related Chemokines during theDevelopment of Rat Mesenteric Lymph Nodes.(2018)Biomed Environ Sci.ul;31(7):507-514. Read more>>
  • Liu Y et al. Isolation and characterization of ovine monocyte-derived macrophages from peripheral blood.(2018)Vet Immunol Immunopathol. Nov;205:83-92. Read more>>
  • Li Y et al. Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats. Mol Med Rep. 2019 Sep 20. Read more>>
  • Ben Menachem-Zidon O et al. Age-associated differences in macrophage response in a vaginal wound healing rat model. Int Urogynecol J. 2020 Feb 27.Read more>>
  • Liu Xingdan. et al. Hydroxyapatite composited PEEK with 3D porous surface enhances osteoblast differentiation through mediating NO by macrophage. Regen Biomater. 2021 DecRead more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy5 conjugated(bs-0295G-Cy5)used at 1:200 dilution


Formalin-fixed and paraffin embedded human laryngocarcinoma labeled with Rabbit Anti-CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Raji probed with CCR7/CD197 Polyclonal Antibody, Unconjugated (bs-1305R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).


THP-1 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CCR7/CD197 Polyclonal Antibody(bs-1305R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).