bs-13074R-A488

DATASHEET

Host: Rabbit

Target Protein: eNOS Ser633

Modification Site: Ser633

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4846

Source: KLH conjugated synthetic phosphopeptide derived from human eNOS around the phosphorylation site of Ser633

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function.

Conjugation: ALEXA FLUOR® 488

Excitation/ Emission: 490nm/525nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 133

Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Sheep
Pig
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.