bs-1313R [Primary Antibody]
VEGFA Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: VEGFA

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7422

Swiss Prot: P15692

Source: KLH conjugated synthetic peptide derived from human VEGF

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.
Function : Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.

Size: 100ul

Concentration: 1ug/ul

Applications: WB()
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Predicted Molecular Weight: 23


Cross Reactive Species: Human
Mouse
Rat
Rabbit

Predicted Cross Reactive Species: Dog
Cow
Chicken
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Gautam et al. Implantation of autologous adipose-derived cells reconstructs functional urethral sphincters in rabbit cryoinjured urethra. (2014) Tissue.Eng.Part.. 20:1971-9Read more>>
  • Li et al. Curcumin attenuates the development of thoracic aortic aneurysm by inhibiting VEGF expression and inflammation. (2017) Mol.Med.Rep. 16:4455-4462Read more>>
  • Ren et al. Interferon-γ and celecoxib inhibit lung-tumor growth through modulating M2/M1 macrophage ratio in the tumor microenvironment. (2014) Drug.Des.Devel.Ther. 8:1527-38Read more>>
  • Chi et al. Transdermal estrogen gel and oral aspirin combination therapy improves fertility prognosis via the promotion of endometrial receptivity in moderate to severe intrauterine adhesion. (2018) Mol.Med.Rep. 17:6337-6344Read more>>
  • Zhen-zhenChen et al. Bu Yang Huan Wu Decoction prevents reperfusion injury following ischemic stroke in rats via inhibition of HIF-1 α, VEGF and promotion β-ENaC expression (2018)Journal of Ethnopharmacology Sep 13;228:70-81.Read more>>
  • Bi SX et al. The antitumour growth and antiangiogenesis effects of xanthatin in murine glioma dynamically evaluated by dynamic contrast‐enhanced magnetic resonance imaging.(2018) Phytother Res. Oct 22.Read more>>
  • Li Z et al. Angiogenesis and bone regeneration by allogeneic mesenchymal stem cell intravenous transplantation in rabbit model of avascular necrotic femoral head. J Surg Res. 2013 Jul;183(1):193-203. Read more>>
  • Wang H et al. The effects of a hirudin/liposome complex on a diabetic nephropathy rat model. BMC Complement Altern Med. 2019 Jun 6;19(1):118.Read more>>
  • Li R et al. Lithium chloride promoted hematoma resolution after intracerebral hemorrhage through GSK-3β-mediated pathways-dependent microglia phagocytosis and M2-phenotype differentiation, angiogenesis and neurogenesis in a rat model. Brain Res Bull. 2019 Jul 17;152:117-127.Read more>>
  • Ma WJ et al. Biliary antibiotics irrigation for E. coli-induced chronic proliferative cholangitis and hepatolithiasis: A pathophysiological study in rabbits. Clin Res Hepatol Gastroenterol. 2019 Aug 13. pii: S2210-7401(19)30173-1.Read more>>
  • Han Y et al. Studies on bacterial cellulose/poly vinyl alcohol (BC/PVA) hydrogel composites as tissue-engineered corneal stroma. Biomed Mater. 2019 Nov 12. Read more>>
  • Liu N et al. Polyphotosensitizer Nanogels for GSH-Responsive Histone Deacetylase Inhibitors delivery and enhanced Cancer Photodynamic Therapy. Colloids Surf B Biointerfaces. 2019 Dec 23;188:110753.Read more>>
  • Zhang Q et al. The circular RNA hsa_circ_0007623 acts as a sponge of microRNA-297 and promotes cardiac repair. Biochem Biophys Res Commun. 2020 Mar 19;523(4):993-1000.Read more>>
  • Yahuafai J et al. Anticancer Efficacy of the Combination of Berberine and PEGylated Liposomal Doxorubicin in Meth A Sarcoma-Bearing Mice.Biol Pharm Bull. 2018;41(7):1103-1106.Read more>>
  • Ding J et al. Anti-angiogenic effect of a chemically sulfated polysaccharide from Phellinus ribis by inhibiting VEGF/VEGFR pathway. Int J Biol Macromol. 2020 Mar 11;154:72-81. Read more>>
  • ?zel F et al. Protective effect of alpha lipoic acid on 4-vinylcyclohexene diepoxide induced primary ovarian failure in female rats. Taiwan J Obstet Gynecol. 2020 Mar;59(2):293-300. Read more>>
  • Ding J et al. Anti-angiogenic effect of a chemically sulfated polysaccharide from Phellinus ribis by inhibiting VEGF/VEGFR pathway. Int J Biol Macromol. 2020 Mar 11;154:72-81. Read more>>
  • Li-Hua Muet al. Antiangiogenic effects of AG36, a triterpenoid saponin from Ardisia gigantifolia stapf. J Nat Med. 2020 Sep;74(4):732-740.Read more>>
  • Hu Y et al. Non-Invasive Estimation of Glioma IDH1 Mutation and VEGF Expression by Histogram Analysis of Dynamic Contrast-Enhanced MRIFront Oncol.2020 Dec 8;10:593102.Read more>>
  • Yanhong Wang. et al. Intermedin Alleviates Renal Ischemia-Reperfusion Injury and Enhances Neovascularization in Wistar Rats. Drug Des Dev Ther. 2020; 14: 4825C4834Read more>>
  • Ismet Hortu. et al. Oxytocin and cabergoline alleviate ovarian hyperstimulation syndrome (OHSS) by suppressing vascular endothelial growth factor (VEGF) in an experimental model. 2020 Nov 02Read more>>
  • Xuewen Qian. et al. Acidosis induces synovial fibroblasts to release vascular endothelial growth factor via acid-sensitive ion channel 1a. Lab Invest. 2020 Aug;101(3):280-291Read more>>
  • Wei P. et al. Nintedanib ameliorates tracheal stenosis by activating HDAC2 and suppressing IL-8 and VEGF in rabbit.. Am J Transl Res. 2020 Aug;12(8):4739-4748Read more>>
  • Yong Tang. et al. Laminin alpha 4 promotes bone regeneration by facilitating cell adhesion and vascularization. Acta Biomater. 2021 MaRead more>>
  • Shun Zhang. et al. PPAR induces the paroxysm of endometriosis by regulating the transcription of MAT2A gene. Am J Transl Res. 2021; 13(3): 1377C1388Read more>>
  • Ling Zhou. et al. Psoriatic mesenchymal stem cells stimulate the angiogenesis of human umbilical vein endothelial cells in vitro. Microvasc Res. 2021 Jul;136:104151Read more>>
  • Qiong Li. et al. CD73+ Mesenchymal Stem Cells Ameliorate Myocardial Infarction by Promoting Angiogenesis. Front Cell Dev Biol. 2021; 9: 637239Read more>>
  • Jian-xia Wen. et al. Therapeutic Effects and Potential Mechanism of Dehydroevodiamine on N-Methyl-N-Nitro-N-Nitrosoguanidine-Induced Chronic Atrophic Gastritis. Phytomedicine. 2021 Jun;:153619Read more>>
  • Huihua Kai. et al. LncRNA NORAD Promotes Vascular Endothelial Cell Injury and Atherosclerosis Through Suppressing VEGF Gene Transcription via Enhancing H3K9 Deacetylation by Recruiting HDAC6. Front Cell Dev Biol. 2021; 9: 701628Read more>>
  • Li Zhi-xin. et al. circGLI3 Inhibits Oxidative Stress by Regulating the miR-339-5p/VEGFA Axis in IPEC-J2 Cells. Biomed Res Int. 2021;2021:1086206Read more>>
  • Ming Ni. et al. Sox11 Modified Tendon-Derived Stem Cells Promote the Repair of Osteonecrosis of Femoral Head:. Cell Transplant. 2021;()Read more>>
  • Liang Hu. et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci. 2021 Jan;22(24):13493Read more>>
  • Lin Zou. et al. Icariin-releasing 3D printed scaffold for bone regeneration. Compos Part B-Eng. 2022 Mar;232:109625Read more>>
  • Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-_B pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166Read more>>
  • Jie Wang. et al. A radiomics model based on DCE-MRI and DWI may improve the prediction of estimating IDH1 mutation and angiogenesis in gliomas. Eur J Radiol. 2022 Feb;147:110141Read more>>
  • Qiuyue Ding. et al. Bioinspired Multifunctional Black Phosphorus Hydrogel with Antibacterial and Antioxidant Properties: A Stepwise Countermeasure for Diabetic Skin Wound Healing. 2022 Feb 19Read more>>
  • Liu, Jingyu. et al. Therapeutic effect of adipose stromal vascular fraction spheroids for partial bladder outlet obstruction induced underactive bladder. Stem Cell Res Ther. 2022 Dec;13(1):1-16Read more>>
  • Shangren Wang. et al. A study of diabetes-induced erectile dysfunction treated with human umbilical cord mesenchymal stem cells. ANDROLOGIA. 2022 Apr 12Read more>>
  • Rui Feng. et al. Progesterone regulates inflammation and receptivity of cells via the NF-_B and LIF/STAT3 pathways. THERIOGENOLOGY. Theriogenology. 2022 Jul;186:5Read more>>
  • Li Pei-Pei. et al. Naoluo Xintong Decoction Ameliorates Cerebral Ischemia-Reperfusion Injury by Promoting Angiogenesis through Activating the HIF-1_/VEGF Signaling Pathway in Rats. EVID-BASED COMPL ALT. 2022;2022:9341466Read more>>
  • Lu, Gonggong. et al. An instantly fixable and self-adaptive scaffold for skull regeneration by autologous stem cell recruitment and angiogenesis. NAT COMMUN. 2022 May;13(1):1-2Read more>>
  • Mei, Jiawei. et al. An injectable photo-cross-linking silk hydrogel system augments diabetic wound healing in orthopaedic surgery through spatiotemporal immunomodulation. J NANOBIOTECHNOL. 2022 Dec;20(1):1-22Read more>>
  • Yu Hedong. et al. Improved repair of rabbit calvarial defects with hydroxyapatite/chitosan/polycaprolactone composite scaffold-engrafted EPCs and BMSCs. FRONT BIOENG BIOTECH. 2022 Aug;0:1275Read more>>
  • Shi Yaxin. et al. Analysis of the Mechanism of GuizhiFuling Wan in Treating Adenomyosis Based on Network Pharmacology Combined with Molecular Docking and Experimental Verification. EVID-BASED COMPL ALT. 2022;2022:6350257Read more>>
  • Huan Lei. et al. A Combination Therapy Using Electrical Stimulation and Adaptive, Conductive Hydrogels Loaded with Self-Assembled Nanogels Incorporating Short Interfering RNA Promotes the Repair of Diabetic Chronic Wounds. Advanced Science. 2022 Sep;:2201425Read more>>
  • Yan LI. et al. The combination of EGCG with warfarin reduces deep vein thrombosis in rabbits through modulating HIF-1 and VEGF via the PI3K/AKT and ERK1/2 signaling pathways. CHIN J NAT MEDICINES. 2022 Sep;20:679Read more>>
  • Lifen Wang. et al. Electroacupuncture attenuates ischemic injury after stroke and promotes angiogenesis via activation of EPO mediated Src and VEGF signaling pathways. PLOS ONE. 2022 Sep;17(9):e027462Read more>>
  • Yongxin Guo. et al. Beneficial Effects of Oleosomes Fused with Human Fibroblast Growth Factor 1 on Wound Healing via the Promotion of Angiogenesis. INT J MOL SCI. 2022 Jan;23(21):13152Read more>>
  • Yanhua Mao. et al. Human amniotic mesenchymal stem cells?promote endometrium regeneration in a rat model of intrauterine adhesion. CELL BIOL INT. 2022 NovRead more>>
  • Lubin Zhou. et al. A self-pumping dressing with in situ modification of non-woven fabric for promoting diabetic wound healing. CHEM ENG J. 2022 Dec;:141108Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Rat lung lysates probed with Anti VEGF Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 24kD. Observed band size: 24kD.


Formalin-fixed and paraffin embedded rabbit liver tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti VEGF Polyclonal Antibody,Unconjugated (bs-1313R) at 1:600 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded rabbit kidney tissue labeled with Anti-VEGF Polyclonal Antibody, Unconjugated (bs-1313R) followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded rabbit meniscuslabeled with Anti-VEGF Polyclonal Antibody, Unconjugated (bs-1313R) followed by conjugation to the secondary antibody and DAPI staining


Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.


Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (VEGFA) Polyclonal Antibody, Unconjugated (bs-1313R) at 1:200 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0295G-AF594) for 90 minutes, and DAPI for nuclei staining.