bs-1315R [Primary Antibody]
Reticulon 4 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Reticulon 4

Specificity: Based on the location of the target sequence this antibody will recognize all isoforms(Nogo-A, Nogo-B, and Nogo-C).

Immunogen Range: 251-357/357


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 68585

Swiss Prot: Q99P72

Source: KLH conjugated synthetic peptide derived from Mouse Reticulon 4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The reticulon protein is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Reticulon 4 regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. It regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. May inhibit BACE1 activity and amyloid precursor protein processing.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 39


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Sheep

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Nogo A+B Polyclonal Antibody, Unconjugated (bs-1315R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Mouse Cerebellum lysates; Lane 2: Rat Cerebellum lysates probed with Reticulon 4/Nogo B Polyclonal Antibody, Unconjugated (bs-1315R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.