VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti-FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n
Paraformaldehyde-fixed, paraffin embedded Mouse intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with FCGBP Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4°C, DAB staining.
Positive sample:Paraformaldehyde-fixed, paraffin embedded (rat intestine, left); Negative sample:Paraformaldehyde-fixed, paraffin embedded (rat lung, right);
Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4°C, followed by secondary antibody and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCGBP) Polyclonal Antibody, Unconjugated (bs-13168R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.