bs-1316R [Primary Antibody]
PDGFBB Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: PDGFBB

Immunogen Range: 151-241/241


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 5155

Source: KLH conjugated synthetic peptide derived from human PDGF-B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Platelet Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B, the dimers being connected by disulfide bonds. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 12


Cross Reactive Species: Human
Mouse
Rat
Chicken

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Cipriani et al. Searching for a good model for systemic sclerosis: the molecular profile and vascular changes occurring in UCD-200 chickens strongly resemble the early phase of human systemic sclerosis. (2016) Arch.Med.Sci. 12:828-43Read more>>
  • Yan Li. et al. Injectable hydrogel with MSNs/microRNA-21-5p delivery enables both immunomodification and enhanced angiogenesis for myocardial infarction therapy in pigs. Sci Adv. 2021 Feb;7(9):eabd674Read more>>
  • Ying Xia. et al. SB431542 alleviates lupus nephritis by regulating B cells and inhibiting the TLR9/TGF1/PDGFB signaling. J AUTOIMMUN. 2022 Oct;132:102894Read more>>
  • Qi Wu. et al. Glut10 restrains neointima formation by promoting SMCs mtDNA demethylation and improving mitochondrial function. TRANSL RES. 2023 MayRead more>>
  • Chengmin Feng. et al. Preparation of healing-promoting and fibrosis-inhibiting asymmetric poly(ethylene glycol-b-L-phenylalanine)/cRGD-modified hyaluronate sponges and their applications in hemorrhage and nasal mucosa repair. INT J BIOL MACROMOL. 2024 Feb;258:128911Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-PDGFBB Polyclonal Antibody, Unconjugated (bs-1316R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-PDGFBB Polyclonal Antibody, Unconjugated (bs-1316R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Lane 1: Human Huvec cell lysates at 30 ug; Lane 2: Human A431 cell lysates at 30 ug; Lane 3: Human MCF-7 cell lysates at 30 ug probed with PDGFBB Polyclonal Antibody, Unconjugated (bs-1316R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.