bs-1318R [Primary Antibody]
IL-5 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: IL-5

Immunogen Range: 56-132/132


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 16191

Swiss Prot: P04401

Source: KLH conjugated synthetic peptide derived from mouse IL-5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Factor that induces terminal differentiation of late-developing B-cells to immunoglobulin secreting cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 13


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu Jun Yan. et al. Interleukin-5-induced eosinophil population improves cardiac function after myocardial infarction. CARDIOVASC RES. 2022 Jul;118(9):2165-2178Read more>>
  • Ara~jo C.D.. et al. Comparison of KIT patterns and infiltration of eosinophils in canine mast cell tumor. ARQ BRAS MED VET ZOO. 2023 Nov;75(6):1047-1054Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse lung tissue labeled with Anti- IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) followed by conjugation to the secondary antibody and DAB staining


Paraformaldehyde-fixed, paraffin embedded Rat liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IL-5 Polyclonal Antibody, Unconjugated (bs-1318R) at 1:200 overnight at 4°C, DAB staining.