bs-13216R [Primary Antibody]
Frizzled 10/CD350 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Frizzled 10/CD350

Specificity: Due to the similarity of this protein to Frizzled 9, there is a chance that this antibody will react with this protein based on homology.

Immunogen Range: 100-150/581

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 11211

Swiss Prot: Q9ULW2

Source: KLH conjugated synthetic peptide derived from human Frizzled 10/CD350

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-Frizzled 10/CD350 Polyclonal Antibody, Unconjugated (bs-13216R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


L1 mouse embryo lysate L2 human colon carcinoma lysates probed with Rabbit Anti-Frizzled 10/CD350 Polyclonal Antibody, Unconjugated (bs-13216R) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 for 90 min at 37˚C.