bs-1324R [Primary Antibody]
CCL5/RANTES Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: CCL5/RANTES

Immunogen Range: 62-91/91


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6352

Swiss Prot: P13501

Source: KLH conjugated synthetic peptide derived from human CCL5

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Background:

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed:1679162, PubMed:13864, PubMed:8525373, PubMed:9516414, PubMed:15923218). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(IHC-P)(1:100-500)
IF(IHC-F)(1:100-500)
IF(ICC)(1:100-500)

Predicted Molecular Weight: 7.4/10


Cross Reactive Species: Human
Mouse
Pig
Guinea Pig

Predicted Cross Reactive Species: Rat
Dog
Cow
Sheep
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Pattappa, G., et al. "CCL5/RANTES is a key chemoattractant released by degenerative intervertebral discs in organ culture." European Cells & Materials 27 (2014): 124.Read more>>
  • Hwaiz, Rundk, et al. "Rac1-dependent secretion of platelet-derived CCL5 regulates neutrophil recruitment via activation of alveolar macrophages in septic lung injury." Journal of Leukocyte Biology (2015): jlb-4A1214.Read more>>
  • Sun, Yubo, et al. "Biological effects and osteoarthritic disease‐modifying activity of small molecule CM‐01." Journal of Orthopaedic Research (2017).Read more>>
  • Li, Yalin, et al. "Batf3-dependent CD8α+ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages." EBioMedicine 18 (2017): 188-198.Read more>>
  • Irie, Koichiro, et al. "Age-related changes of CD4+ T cell migration and cytokine expression in germ-free and SPF mice periodontium." Archives of oral biology (2017).Read more>>
  • Sun et al. Disease-modifying effects of phosphocitrate and phosphocitrate-β-ethyl ester on partial meniscectomy-induced osteoarthritis. (2015) BMC.Musculoskelet.Disord. 16:270Read more>>
  • Linardi Daniele. et al. Slow versus fast rewarming after hypothermic circulatory arrest: effects on neuroinflammation and cerebral oedema. Eur J Cardio-Thorac. 2020 Oct;58(4):792-8Read more>>
  • ing Han. et al. Pulmonary inflammatory and fibrogenic response induced by graphitized multi-walled carbon nanotube involved in cGAS-STING signaling pathway. J Hazard Mater. 2021 May;:125984Read more>>
  • Yi Wu. et al. Targeting cIAPs attenuates CCl4-induced liver fibrosis by increasing MMP9 expression derived from neutrophils. Life Sci. 2022 Jan;289:120235Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-CCL5/RANTES Polyclonal Antibody, Unconjugated (bs-1324R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.