bs-13255R

DATASHEET

Host: Rabbit

Target Protein: GAB3

Immunogen Range: 31-130/586

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 139716

Source: KLH conjugated synthetic peptide derived from human GAB3

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

The Gab family of adaptor proteins function as molecular scaffolds that mediate protein recrutiment to RTKs (13). Cytokine/growth factor triggering of protein tyrosine kinase receptors (RTKs) initiates signaling cascades that progress to the nucleus where signals for activation, proliferation and differentiation occur (13). This scaffolding mechanism represents a critical link in cytokine/growth factor signaling routes. Gab1-3 contain pleckstrin homology and potential binding sites for SH2 and SH3 domain-containing proteins (1,4,5). The recruitment of signaling partners to Gab family members is phosphorylation dependent (1,4). Insulin receptor and EGF-receptor signaling are among the cascades that rely on Gab family members to elicit a nuclear response to an extracellular stimulus (6,7). The human Gab3 gene maps to chromosome Xq28 and encodes a 586 amino acid protein (5).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 66

Predicted Cross Reactive Species: Human
Mouse
Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.