bs-1335R [Primary Antibody]
Notch1 Polyclonal Antibody
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Host: Rabbit

Target Protein: Notch1

Immunogen Range: 2101-2300/2555


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4851

Swiss Prot: P46531

Source: KLH conjugated synthetic peptide derived from human Notch1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 86/89/271


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zou et al. Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway. (2018) EBioMedicine. 31:287-298Read more>>
  • Bulnes et al. Association of Notch-1, osteopontin and stem-like cells in ENU-glioma malignant process. (2018) Oncotarget. 9:31330-31341Read more>>
  • "Chen X et al. A photothermal-triggered nitric oxide nanogenerator combined with siRNA for precise therapy of osteoarthritis by suppressing macrophage inflammation.Nanoscale. 2019 Apr 4;11(14):6693-6709."Read more>>
  • Zhang Y et al. The Notch signaling pathway inhibitor Dapt alleviates autism-like behavior, autophagy and dendritic spine density abnormalities in a valproic acid-induced animal model of autism. Prog Neuropsychopharmacol Biol Psychiatry. 2019 May 7;94:109644. Read more>>
  • Zhan J et al. In Vivo Study on the Effects of Xiaoaiping on the Stemness of Hepatocellular Carcinoma Cells. Evid Based Complement Alternat Med. 2019 Jun 23;2019:4738243.Read more>>
  • Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
  • Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
  • Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
  • Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
  • Hisashi Yoshioka. et al. Senescent cell removal via JAG1\NOTCH1 signalling in the epidermis. 2021 May 03Read more>>
  • enda L. K. Coles. et al. A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells. Lab Chip. 2021 OcRead more>>
  • enda L. K. Coles. et al. A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells. Lab Chip. 2021 OcRead more>>
  • Ying-Hua Zhang. et al. N-acetylcysteine improves autism-like behavior by recovering autophagic deficiency and decreasing Notch-1/Hes-1 pathway activity. EXP BIOL MED. ;()Read more>>
  • Sun Shin. et al. Leprosy-specific subsets of macrophages and Schwann cells identified by single-cell RNA-sequencing. PATHOL RES PRACT. 2023 Oct;250:154821Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Notch1 Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Notch1 Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Notch1 Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Notch1) polyclonal Antibody, Unconjugated (bs-1335R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Notch1 Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with Notch1 polyclonal antibody, unconjugated (bs-1335R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.


Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Notch1 Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.


A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Notch1) polyclonal Antibody, Unconjugated (bs-1335R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.


Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Notch1 Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.