bs-1335R
[Primary Antibody]
Notch1/MOTC Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Notch1/MOTC
Immunogen Range: 2101-2300/2555
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4851
Swiss Prot: P46531
Source: KLH conjugated synthetic peptide derived from human Notch1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 86/89/271
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zou et al. Hydroxylase Activity of ASPH Promotes Hepatocellular Carcinoma Metastasis Through Epithelial-to-Mesenchymal Transition Pathway. (2018) EBioMedicine. 31:287-298Read more>>
- Bulnes et al. Association of Notch-1, osteopontin and stem-like cells in ENU-glioma malignant process. (2018) Oncotarget. 9:31330-31341Read more>>
- "Chen X et al. A photothermal-triggered nitric oxide nanogenerator combined with siRNA for precise therapy of osteoarthritis by suppressing macrophage inflammation.Nanoscale. 2019 Apr 4;11(14):6693-6709."Read more>>
- Zhang Y et al. The Notch signaling pathway inhibitor Dapt alleviates autism-like behavior, autophagy and dendritic spine density abnormalities in a valproic acid-induced animal model of autism. Prog Neuropsychopharmacol Biol Psychiatry. 2019 May 7;94:109644. Read more>>
- Zhan J et al. In Vivo Study on the Effects of Xiaoaiping on the Stemness of Hepatocellular Carcinoma Cells. Evid Based Complement Alternat Med. 2019 Jun 23;2019:4738243.Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
- Wang EX et al. Stem cells from trabecular meshwork cells can secrete extracellular matrix. Biochem Biophys Res Commun. 2020 Mar 5;523(2):522-526.Read more>>
- Yong Tang. et al. Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering. Bioact Mater. 2021 Jul;6:2039Read more>>
- Hisashi Yoshioka. et al. Senescent cell removal via JAG1\NOTCH1 signalling in the epidermis. 2021 May 03Read more>>
- enda L. K. Coles. et al. A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells. Lab Chip. 2021 OcRead more>>
- enda L. K. Coles. et al. A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells. Lab Chip. 2021 OcRead more>>
- Ying-Hua Zhang. et al. N-acetylcysteine improves autism-like behavior by recovering autophagic deficiency and decreasing Notch-1/Hes-1 pathway activity. EXP BIOL MED. ;()Read more>>
- Sun Shin. et al. Leprosy-specific subsets of macrophages and Schwann cells identified by single-cell RNA-sequencing. PATHOL RES PRACT. 2023 Oct;250:154821Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded human endometrial carcinoma labeled with Anti-Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, DAB staining.
Lane 1: Human MCF-7 cell lysates; Lane 2: Human A549 cell lysates probed with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human MOLT-4 cell lysates; Lane 2: Human MCF-7 cell lysates probed with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Notch1/MOTC Polyclonal Antibody, Unconjugated (bs-1335R) at 1:200 overnight at 4°C, DAB staining.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Notch1) polyclonal Antibody, Unconjugated (bs-1335R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Notch1) polyclonal Antibody, Unconjugated (bs-1335R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.