bs-13378R [Primary Antibody]
COLGALT1 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: COLGALT1

Immunogen Range: 531-622/622


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 79709

Swiss Prot: Q8NBJ5

Source: KLH conjugated synthetic peptide derived from human GLT25D1

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

Background:

GLT25D1 is a 622 amino acid protein that localizes to the lumen of the endoplasmic reticulum. Ubiquitously expressed with higher levels in placenta, heart, lung and spleen, GLT25D1 is a member of the glycosyltransferase 25 family. The beta-galactosyltransferase activity of GLT25D1 allows the transfer of beta-galactose to hydroxylysine residues of collagen. GLT25D1 is considered an important target for investigating the biological significance of collagen glycosylation and the importance of posttranslational modification in the etiology of connective tissue disorders. The gene encoding GLT25D1 is located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
Flow-Cyt(1ug/Test)

Predicted Molecular Weight: 69


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Dog
Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Mouse Placenta tissue lysates; Lane 2: Mouse Liver tissue lysates; Lane 3: Rat Placenta tissue lysates; Lane 4: Rat Spleen tissue lysates; Lane 5: Rat Liver tissue lysates; Lane 6: Human THP-1 cell lysates probed with COLGALT1 /GLT25D1Polyclonal Antibody, Unconjugated (bs-13378R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.