Host: Rabbit
Target Protein: NRF-1
Immunogen Range: 51-180/503
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4899
Swiss Prot: Q16656
Source: KLH conjugated synthetic peptide derived from human NRF1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 55
Cross Reactive Species:
Human
Mouse
Rat
For research use only. Not intended for diagnostic or therapeutic use.
- Qin, Guohua, et al. "Sulfur dioxide inhalation stimulated mitochondrial biogenesis in rat brains." Toxicology 300.1 (2012): 67-74.Read more>>
- Yan, Wei, et al. "Acute nitrogen dioxide inhalation induces mitochondrial dysfunction in rat brain." Environmental Research 138 (2015): 416-424.Read more>>
- Yue Shen. et al. Lycopene prevents Di-(2-ethylhexyl) phthalate-induced mitophagy and oxidative stress in mice heart via modulating mitochondrial homeostasis. J NUTR BIOCHEM. 2023 May;115:109285Read more>>
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti NRF-1 Polyclonal Antibody, Unconjugated (bs-1342R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Mouse heart and muscle lysates probed with Rabbit Anti-NRF-1 Polyclonal Antibody, Unconjugated (bs-1342R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Lane 1: mouse lung lysates Lane 2: human colon carcinoma lysates probed with Anti-NRF-1 Polyclonal Antibody, Unconjugated (bs-1342R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band 55kD. Observed band size: 55kD.
Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NRF1) polyclonal Antibody, Unconjugated (bs-1342R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.