bs-1343R [Primary Antibody]
Ezrin Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Ezrin

Immunogen Range: 455-586/586


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 7430

Swiss Prot: P15311

Source: KLH conjugated synthetic peptide derived from human Ezrin

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 69


Cross Reactive Species: Human
Rat

Predicted Cross Reactive Species: Mouse
Dog
Cow
Pig
Horse
Rabbit
Guinea Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zhao, Ran, et al. "Hepatoma cell line HepG2. 2.15 demonstrates distinct biological features compared with parental HepG2." World journal of gastroenterology: WJG 17.9 (2011): 1152.Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human endometrium carcinoma labeled with Anti-Ezrin Polyclonal Antibody, Unconjugated (bs-1343R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


RSC96 probed with Ezrin Polyclonal Antibody, Unconjugated (bs-1343R) at 1:100 for 30 minutes followed by incubation with a PE conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).