bs-13451R [Primary Antibody]
GLYR1/NP60 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: GLYR1/NP60

Immunogen Range: 21-120/553


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 84656

Source: KLH conjugated synthetic peptide derived from human NP60

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

NP60 is a 553 amino acid nuclear protein that regulates the phosphorylation and activation of p38 alpha in response to stress. There are five isoforms of NP60 that are produced as a result of alternative splicing events.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 61


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Chicken
Monkey

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Lane 1: Liver lysates probed with GLYR1/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Uterus lysates probed with GLYR1/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.


Lane 1: Hela cell lysates; Lane 2: Mouse Stomach lysates; Lane 3: Mouse Liver lysates; Lane 4: Mouse Kidney lysates probed with GLYR1/NP60 Polyclonal Antibody, Unconjugated (bs-13451R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.