bs-13487R [Primary Antibody]
Golgin subfamily A member 4/Golgin 245 Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
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DATASHEET

Host: Rabbit

Target Protein: Golgin subfamily A member 4/Golgin 245

Immunogen Range: 2061-2160/2230


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 2803

Source: KLH conjugated synthetic peptide derived from human Golgin subfamily A member 4/Golgin 245

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Golgin 245 is also known as p230, GCP2, GOLG or golgi autoantigen and is a 2,230 amino acid protein that is expressed as three isoforms. Golgin 245 is localized to the cytoplasm in cells and is a member of the golgin family, all of which are peripheral membrane proteins associated with the Golgi complex. Golgin 245 has a carboxyl-terminal GRIP domain, which attaches to the trans-Golgi network (TGN) and TGN-derived vesicles. It is thought that the interaction that takes place between golgin 245 and MACF1, which cross-links microtubules to the Actin cytoskeleton, allows proteins to be transported from the TGN to the cell periphery. against golgin 245 are associated with two chronic disorders known as Sjren's syndrome and Hepatitis B.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 261


Cross Reactive Species: Mouse

Predicted Cross Reactive Species: Human
Rat
Cow
Pig
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin-embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GOGA4) Polyclonal Antibody, Unconjugated (bs-13487R) at 1:400 overnight at 4°C, followed by DAB staining.