Host: Rabbit
Target Protein: Granzyme B & Granzyme H
Specificity: Specific towards Granzyme B and Granzyme H
Immunogen Range: 21-130/247
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 3002
Swiss Prot: P10144
Source: KLH conjugated synthetic peptide derived from human GZMB
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 25
Cross Reactive Species:
Human
Mouse
Others
Predicted Cross Reactive Species:
Rat
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
- Oláh, Zita, et al. "Proteomic Analysis of Cerebrospinal Fluid in Alzheimers Disease: Wanted Dead or Alive." Journal of Alzheimers Disease (2014).Read more>>
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Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-Granzyme B Polyclonal Antibody, Unconjugated (bs-1351R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Antigen: bs-1351P, 0.2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; \nTMB staining; Read the data in MicroplateReader by 450nm. \n
U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1% PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Granzyme B Antibody(bs-1351R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Human K562 cell lysates; Lane 2: Human MOLT4 cell lysates probed with Granzyme B Polyclonal Antibody, Unconjugated (bs-1351R) at 1:500 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.