bs-1351R-Cy5 [Conjugated Primary Antibody]
Pan Granzyme Polyclonal Antibody, Cy5 Conjugated
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Granzyme B & Granzyme H

Specificity: Specific towards Granzyme B and Granzyme H

Immunogen Range: 21-130/247


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 3002

Swiss Prot: P10144

Source: KLH conjugated synthetic peptide derived from human GZMB

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Conjugation: Cy5

Excitation/ Emission: 625,650nm/670nm

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
FCM(1:20-100)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 25


Cross Reactive Species: Human
Mouse
Others

Predicted Cross Reactive Species: Rat
Cow
Pig

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Oláh, Zita, et al. "Proteomic Analysis of Cerebrospinal Fluid in Alzheimers Disease: Wanted Dead or Alive." Journal of Alzheimers Disease (2014).Read more>>
  • Mu JY et al. Astragalus polysaccharide restores activation of NK cells in radiation therapy of tumors. Int J Clin Exp Med 2019;12(7):8609-8621.Read more>>
  • Shuling Zhang. et al. Methionine enkephalin (MENK) suppresses lung cancer by regulating the Bcl-2/Bax/caspase-3 signaling pathway and enhancing natural killer cell-driven tumor immunity. Int Immunopharmacol. 2021 Sep;98:107837Read more>>
  • Ji Eun Won. et al. Immune checkpoint silencing using RNAi-incorporated nanoparticles enhances antitumor immunity and therapeutic efficacy compared with antibody-based approaches. J Immunother Cancer. 2022 Feb;10(2):e003928Read more>>
  • Wenjing Guo. et al. Self-Assembly of a Multifunction DNA Tetrahedron for Effective Delivery of Aptamer PL1 and Pcsk9 siRNA Potentiate Immune Checkpoint Therapy for Colorectal Cancer. ACS APPL MATER INTER. 2022;14(28):3163431644Read more>>
  • Wu Yutong. et al. Osteoclast-Derived Apoptotic Bodies Inhibit Naive Cd8<sup> </sup> T Cell Activation via Siglec15 Promoting Breast Cancer Secondary Metastasis. Cell Reports Medicine. 2022 Nov 03Read more>>
  • El-Badawy Omnia. et al. Cross-Talk between Mucosal-Associated Invariant T, Natural Killer, and Natural Killer T Cell Populations is Implicated in the Pathogenesis of Placenta Accreta Spectrum. INFLAMMATION. 2023 Mar;:1-17Read more>>
  • Huang Xinkun. et al. CHSY3 promotes proliferation and migration in gastric cancer and is associated with immune infiltration. J TRANSL MED. 2023 Dec;21(1):1-2Read more>>
  • Junfeng Zhao. et al. Administration of a glypican-3 peptide increases the infiltration and cytotoxicity of CD8+ T cells against testicular yolk sac tumor, associated with enhancing the intratumoral cGAS/STING signaling. CANCER MED-US. 2023 NovRead more>>
VALIDATION IMAGES