bs-13536R
[Primary Antibody]
GPR43 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GPR43
Immunogen Range: 41-140/330
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2867
Swiss Prot: O15552
Source: KLH conjugated synthetic peptide derived from human GPR43
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
G protein-coupled receptor that is activated by a major product of dietary fiber digestion, the short chain fatty acids (SCFAs), and that plays a role in the regulation of whole-body energy homeostasis and in intestinal immunity. In omnivorous mammals, the short chain fatty acids acetate, propionate and butyrate are produced primarily by the gut microbiome that metabolizes dietary fibers. SCFAs serve as a source of energy but also act as signaling molecules. That G protein-coupled receptor is probably coupled to the pertussis toxin-sensitive, G(i/o)-alpha family of G proteins but also to the Gq family (PubMed:12496283, PubMed:12711604, PubMed:23589301). Its activation results in the formation of inositol 1,4,5-trisphosphate, the mobilization of intracellular calcium, the phosphorylation of the MAPK3/ERK1 and MAPK1/ERK2 kinases and the inhibition of intracellular cAMP accumulation. May play a role in glucose homeostasis by regulating the secretion of GLP-1, in response to short-chain fatty acids accumulating in the intestine. May also regulate the production of LEP/Leptin, a hormone acting on the central nervous system to inhibit food intake. Finally, may also regulate whole-body energy homeostasis through adipogenesis regulating both differentiation and lipid storage of adipocytes. In parallel to its role in energy homeostasis, may also mediate the activation of the inflammatory and immune responses by SCFA in the intestine, regulating the rapid production of chemokines and cytokines. May also play a role in the resolution of the inflammatory response and control chemotaxis in neutrophils. In addition to SCFAs, may also be activated by the extracellular lectin FCN1 in a process leading to activation of monocytes and inducing the secretion of interleukin-8/IL-8 in response to the presence of microbes (PubMed:21037097).
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
Predicted Molecular Weight: 31
Cross Reactive Species:
Mouse
Rat
Predicted Cross Reactive Species: Human
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Li T et al. Bovine α-lactalbumin hydrolysates ameliorate obesity-associated endotoxemia and inflammation in high-fat diet-fed mice through modulation of gut microbiota. Food Funct. 2019 May 17.Read more>>
- Kondo T et al. Prebiotic effect of fructooligosaccharides on the inner ear of DBA/2 J mice with early-onset progressive hearing loss. The Journal of Nutritional Biochemistry. 2019 September.Read more>>
- Hu ZB et al. Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis. Theranostics. 2020 Feb 3;10(6):2803-2816.Read more>>
- Hu ZB et al. Dysbiosis of intestinal microbiota mediates tubulointerstitial injury in diabetic nephropathy via the disruption of cholesterol homeostasis. Theranostics. 2020 Feb 3;10(6):2803-2816.Read more>>
- Wen Xianghui. et al. Qu-Zhuo-Tong-Bi Decoction Alleviates Gouty Arthritis by Regulating Butyrate-Producing Bacteria in Mice. Front Pharmacol. 2021 Feb;11:2508Read more>>
- Guangning Kou. et al. Nobiletin activates thermogenesis of brown and white adipose tissue in high\fat diet\fed C57BL/6 mice by shaping the gut microbiota. Faseb J. 2021 Feb;35(2):e21267Read more>>
- Gang Tang. et al. Butyrate ameliorate skeletal muscle atrophy in Diabetic Nephropathy via enhancing gut barrier function and FFA2-mediated PI3K/AKT/mTOR signals. 2021 Oct 12Read more>>
- Yen-Chieh Wang. et al. Supplementation of Probiotic Butyricicoccus pullicaecorum Mediates Anticancer Effect on Bladder Urothelial Cells by Regulating Butyrate-Responsive Molecular Signatures. Diagnostics. 2021 Dec;11(12):227Read more>>
- Christina Fischer. et al. Propionate induces cross-tolerance to TLR1/2 and TLR4 agonists in an IFIT-dependent manner. Immunobiology. 2022 Mar;227:152186Read more>>
- Yao Yao. et al. Short-chain fatty acids regulate B cells differentiation via FFAR2 to alleviate rheumatoid arthritis. BRIT J PHARMACOL. 2022 Apr 07Read more>>
- Fujinaga, Atsuro. et al. Changes of Short-Chain Fatty Acids and Their Receptors in an Obese Rat Model After Sleeve Gastrectomy. OBES SURG. 2022 Jun;:1-9Read more>>
- Chun Niu. et al. Pulsatilla decoction improves DSS-induced colitis via modulation of fecal-bacteria-related short-chain fatty acids and intestinal barrier integrity. J ETHNOPHARMACOL. 2023 Jan;300:115741Read more>>
- Kaidong Wang. et al. Gut dysfunction may be the source of pathological aggregation of alpha-synuclein in the central nervous system through Paraquat exposure in mice. ECOTOX ENVIRON SAFE. 2022 Nov;246:114152Read more>>
- Tomoaki NAITO. et al. Diverse impact of a probiotic strain, Lacticaseibacillus paracasei Shirota, on peripheral mononuclear phagocytic cells in healthy Japanese office workers: a randomized, double-blind, controlled trial. Bioscience of Microbiota Food and Health. 2022 Oct 11Read more>>
- Yu Zhou. et al. Liangxue Tongyu Prescription Alleviates Brain Damage in Acute Intracerebral Hemorrhage Rats by Regulating Intestinal Mucosal Barrier Function. EVID-BASED COMPL ALT. 2022 Dec 17;2022:2197763Read more>>
- Niu, Junling. et al. Microbiota-derived acetate enhances host antiviral response via NLRP3. NAT COMMUN. 2023 Feb;14(1):1-17Read more>>
- Yu-yan Gu. et al. Dingxin recipe ameliorates hyperlipidemia injury in SD rats by improving the gut barrier, particularly the SCFAs/GPR43 pathway. J ETHNOPHARMACOL. 2023 Apr;:116483Read more>>
- Xueran Mei. et al. Maternal Phlorizin Intake Protects Offspring from Maternal Obesity-Induced Metabolic Disorders in Mice via Targeting Gut Microbiota to Activate the SCFA-GPR43 Pathway. J AGR FOOD CHEM. 2024;XXXX(XXX):XXX-XXXRead more>>
- Cuirong Zeng. et al. B-GOS alleviates olanzapine-induced lipid disturbances in mice by enriching Akkermansia and upregulation of PGRMC1-Wnt signaling. FOOD CHEM TOXICOL. 2024 Mar;185:11449Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded mouse spleen labeled with Anti-GPR43 Polyclonal Antibody, Unconjugated (bs-13536R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Lane 1: Mouse Stomach lysates; Lane 2: Rat Stomach lysates; Lane 3: Rat Small intestine lysates probed with GPR43 Polyclonal Antibody, Unconjugated (bs-13536R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
THP1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with GPR43 Polyclonal Antibody(bs-13536R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).