bs-13588R [Primary Antibody]
ZNF575 Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: ZNF575

Immunogen Range: 51-150/245


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 284346

Source: KLH conjugated synthetic peptide derived from human ZNF575

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kr_ppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger protein 575 (ZNF575) is a 245 amino acid member of the Kr_ppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZNF575 contains six C2H2-type zinc fingers through which it is thought to be involved in DNA-binding and transcriptional regulation.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 27


Cross Reactive Species: Rat

Predicted Cross Reactive Species: Human
Mouse
Dog
Cow
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • NING AN. et al. The zinc figure protein ZNF575 impairs colorectal cancer growth via promoting p53 transcription. ONCOL RES. 2023; 31(3): 307C316Read more>>
VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded Rat testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ZNF575 Polyclonal Antibody, Unconjugated (bs-13588R) at 1:200 overnight at 4°C, DAB staining.