bs-13638R [Primary Antibody]
AP1M2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: AP1M2

Immunogen Range: 1-100/423


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 10053

Swiss Prot: Q9Y6Q5

Source: KLH conjugated synthetic peptide derived from human AP1M2

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 48


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Cow
Sheep
Pig
Horse

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Mouse small intestine lysates probed with AP1M2 Polyclonal Antibody, unconjugated (bs-13638R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.


Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti-AP1M2 Polyclonal Antibody, Unconjugated (bs-13638R) at 1:200 followed by conjugation to the secondary antibody and DAB staining


Lane 1: Human HepG2 cell lysates; Lane 2: Human MCF-7 cell lysates; Lane 3: Human A431 cell lysates probed with AP1M2 Polyclonal Antibody, Unconjugated (bs-13638R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.