bs-1363R
[Primary Antibody]
AIF1 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: AIF1
Immunogen Range: 51-147/147
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 199
Swiss Prot: P55008
Source: KLH conjugated synthetic peptide derived from human AIF1
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 16
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species: Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Hsu, Shih-Tien, et al. "Effects of Taxol on Regeneration in a Rat Sciatic Nerve Transection Model." Scientific Reports 7 (2017): 42280.Read more>>
- Fu, Juanli, et al. "The acute exposure of tetrachloro-p-benzoquinone (aka chloranil) triggers inflammation and neurological dysfunction via Toll-like receptor 4 signaling: The protective role of melatonin preconditioning." Toxicology (2017).Read more>>
- Song, Eric, et al. "Surface chemistry governs cellular tropism of nanoparticles in the brain." Nature Communications (2017).Read more>>
- Hu. et al. Levo-Corydalmine Alleviates Neuropathic Cancer Pain Induced by Tumor Compression via the CCL2/CCR2 PathwayRead more>>
- Ko, Chien-Hsin, et al. "Biodegradable Bisvinyl Sulfonemethyl-crosslinked Gelatin Conduit Promotes Regeneration after Peripheral Nerve Injury in Adult Rats." Scientific reports 7.1 (2017): 17489.Read more>>
- Furukawa et al. Databases for technical aspects of immunohistochemistry. (2017) J.Toxicol.Patho. 30:79-107Read more>>
- Kouam et al. Induction of Mkp-1 and Nuclear Translocation of Nrf2 by Limonoids from Khaya grandifoliola C.DC Protect L-02 Hepatocytes against Acetaminophen-Induced Hepatotoxicity. (2017) Front.Pharmacol. 8:653Read more>>
- Ji et al. TFP5 peptide, derived from CDK5-activating cofactor p35, provides neuroprotection in early-stage of adult ischemic stroke. (2017) Sci.Rep. 7:40013Read more>>
- Yue J et al. Activation of leukocyte immunoglobulin-like receptor B2 signaling pathway in cortical lesions of pediatric patients with focal cortical dysplasia type IIb and tuberous sclerosis complex. Brain Dev. 2019 Sep 5. pii: S0387-7604(19)30240-2. Read more>>
- Liao CF et al. Effects of endogenous inflammation signals elicited by nerve growth factor, interferon-γ, and interleukin-4 on peripheral nerve regeneration. J Biol Eng. 2019 Nov 13;13:86. Read more>>
- Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. Read more>>
- Yang W et al. The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice. Clin Immunol. 2020 Jan 23;212:108350. Read more>>
- Fange Kong. et al. Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-B signaling in Alzheimers disease. J Neuroinflamm. 2020 Dec;17(1):1-15Read more>>
- Dingkang Xu. et al. Sphingosine\1\phosphate receptor 3 is implicated in BBB injury via the CCL2\CCR2 axis following acute intracerebral hemorrhage. 2021 Feb 28Read more>>
- Lushuang Xie. et al. Inflammatory factors and amyloid -induced microglial polarization promote inflammatory crosstalk with astrocytes.. Aging-Us. 2020 Nov 30; 12(22): 22538C22549Read more>>
- Pengfei Fu. et al. Baicalein Ameliorates Epilepsy Symptoms in a Pilocarpine-Induced Rat Model by Regulation of IGF1R. Neurochem Res. 2020 Dec;45(12):3021-3033Read more>>
- Yanan Shen. et al. Phosphatase and Tensin Homolog Deleted on Chromosome Ten Knockdown Attenuates Cognitive Deficits by Inhibiting Microglia-mediated Neuroinflammation in the Hippocampus in a Mouse Model of Perioperative Neurocognitive Disorder. Neuroscience. 2021 Aug;468:199Read more>>
- Shingo MIYAZAKI. et al. Spontaneous adenocarcinoma with giant cell formation in the accessory sex glands in a male Sprague-Dawley rat. 2021 Jul 23Read more>>
- Tong Wang. et al. Reactive astrocytes induced by 2-chloroethanol modulate microglia polarization through IL-1, TNF-, and iNOS upregulation. Food Chem Toxicol. 2021 Sep;:11255Read more>>
- Meilan Xue. et al. Neuroprotective effect of fucoidan by regulating gut-microbiota-brain axis in alcohol withdrawal mice. J Funct Foods. 2021 Nov;86:104726Read more>>
- Liang Hu. et al. Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages. Int J Mol Sci. 2021 Jan;22(24):13493Read more>>
- Haiyang Zhang. et al. Co-exposure of chronic stress and alumina nanoparticles aggravates hippocampal microglia pyroptosis by activating cathepsin B/NLRP3 signaling pathway. J HAZARD MATER. 2022 Aug;436:129093Read more>>
- Takei, Yoshinori. et al. Alteration in peritoneal cells with the chemokine CX3CL1 reverses age-associated impairment of recognition memory. GEROSCIENCE. 2022 May;:1-14Read more>>
- Wei, Ying. et al. Astrocyte Activation, but not Microglia, Is Associated with the Experimental Mouse Model of Schizophrenia Induced by Chronic Ketamine. J MOL NEUROSCI. 2022 Jul;:1-14Read more>>
- Chuanjiang He. et al. MiRNA-494 specifically inhibits SIRT3-mediated microglia activation in sepsis-associated encephalopathy. TRANSL CANCER RES. 2022 Jul; 11(7): 22992309Read more>>
- Qinrui Hu. et al. Neuroprotection of boropinol-B in Cerebral Ischemia-reperfusion Injury by Inhibiting Inflammation and Apoptosis. BRAIN RES. 2022 Oct;:148132Read more>>
- Yuxin Liu. et al. Ferromagnetic flexible electronics for brain-wide selective neural recording. ADV MATER. 2022 Nov;:2208251Read more>>
- Can Sezer. et al. Neuroprotective Effects of Milrinone on Acute Traumatic Brain ?njury. WORLD NEUROSURG. 2022 NovRead more>>
- Wang Shang. et al. Early activation of Toll-like receptor-3 reduces the pathological progression of Alzheimers disease in APP/PS1 mouse. ALZHEIMERS RES THER. 2023 Dec;15(1):1-17Read more>>
- Guang-Sheng Li. et al. Neurovascular Unit Compensation from Adjacent Level May Contribute to Spontaneous Functional Recovery in Experimental Cervical Spondylotic Myelopathy. INT J MOL SCI. 2023 Jan;24(4):3408Read more>>
- Longcong Dong. et al. Shared Genes of PPARG and NOS2 in Alzheimer’s Disease and Ulcerative Colitis Drive Macrophages and Microglia Polarization: Evidence from Bioinformatics Analysis and Following Validation. INT J MOL SCI. 2023 Jan;24(6):5651Read more>>
- Shan Luo. et al. Application of omics technology to investigate the mechanism underlying the role of San Hua Tang in regulating microglia polarization and blood-brain barrier protection following ischemic stroke. J ETHNOPHARMACOL. 2023 May;:11664Read more>>
- Quancheng Cheng. et al. Integrated multiomics analysis reveals changes in liver physiological function in Aqp9 gene knockout mice. INT J BIOL MACROMOL. 2023 Jun;:125459Read more>>
- Linna Sun. et al. Construction of Curcumin-Loaded Hydrogels for Treatment of Traumatic Brain Injury. ACS APPL POLYM MATER. 2023;XXXX(XXX):XXX-XXXRead more>>
- Xue Zhao. et al. Neural Stem Cell-Derived Exosomes Improve Neurological Function in Rats with Cerebral Ischemia-Reperfusion Injury by Regulating Microglia-Mediated Inflammatory Response. J INFLAMM RES. 2023 Jul 23Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.\n
Image by provided by the Independent Validation program, badge number 029613:Frozen,OCT embedded mouse spleen labeled with Anti-AIF1 Polyclonal Antibody, Unconjugated (bs-1363R) at 1:100 followed by conjugation to the secondary antibody and DAPI staining
Formalin-fixed and paraffin-embedded rat brain labeled with AIF1 Polyclonal Antibody at 1:200 dilution followed by incubation with secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated (bs-1363R) at 1:200 for 40 minutes at 37°C followed by labeling Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3) 1:200, 40 minutes at 37°C
Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. (a) Sham group; (b) Tumor group; (c) l-CDL 5 mg/kg group; (d) l-CDL 10 mg/kg group; (e) l-CDL 20 mg/kg group; (f) O&A 1&65 m/kg group (g) l-THP 20 mg/kg group. B Graph showing the mean fluorescence intensity for AIF1.
BV-microglia stained with AIF1 Polyclonal Antibody (bs-1363R) at 1:100 dilution. AIF1 expression (green) in activated BV-microglia is observed using confocal microscopy. The blue staining represents DAPI. (a) Control; (b) treated with CCL2 (3 ng/mL); (c) CCL2 (3 ng/mL) + l-CDL (3 µM); (d) CCL2 (3 ng/mL) + l-CDL (10 µM); (e) CCL2 (3 ng/mL) + l-CDL (30 µM); (f) CCL2 (3 ng/mL) + RS504393 (10 µM);
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AIF1) Polyclonal Antibody, Unconjugated (bs-1363R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.