bs-13640R-Biotin

DATASHEET

Host: Rabbit

Target Protein: GRAP

Immunogen Range: 51-150/217

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human GRAP

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific receptor sequences. For instance, the GRB family of proteins lack a defined catalytic activity and are entirely composed of SH2 and SH3 domains. Members include GRB2, GRB7, GRB10 (also referred to as GRB-IR), GRB14 and Grap (for GRB2-related adapter protein). While GRB10 and GRB14 are most closely related to GRB7, Grap shares the highest degree of homology with GRB2 exhibiting 59% sequence identity with GRB2. The Grap SH2 domain is capable of binding to the activated stem cell factor receptor, c-Kit and the erythropoietin receptor (EpoR). Grap also associates with the Ras guanine nucleotide exchange factor Sos 1 via its amino terminal SH3 domain.

Conjugation: Biotin

Excitation/ Emission: N/A

Size: 100µL

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)

Predicted Molecular Weight: 25

Predicted Cross Reactive Species: Human
Mouse
Rat
Cow
Horse
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.