bs-1365R
[Primary Antibody]
Tsg101 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: Tsg101
Immunogen Range: 4-120/390
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 7251
Swiss Prot: Q99816
Source: KLH conjugated synthetic peptide derived from human Tsg101
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Background:
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 44
Cross Reactive Species:
Human
Rat
Predicted Cross Reactive Species:
Mouse
Dog
Cow
Horse
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Chen Y et al. The role of infectious hematopoietic necrosis virus (IHNV) proteins in recruiting the ESCRT pathway through three ways in the host cells of fish during IHNV budding. Fish Shellfish Immunol. 2019 Jul 9;92:833-841.Read more>>
- Sun Z et al. Glioblastoma Stem Cell-Derived Exosomes Enhance Stemness and Tumorigenicity of Glioma Cells by Transferring Notch1 Protein. Cell Mol Neurobiol. 2019 Dec 18. Read more>>
- Zhang M et al. Proteomic analysis reveals proteins and pathways associated with lactation in bovine mammary epithelial cell-derived exosomes. J Proteome Res. 2020 Aug 7;19(8):3211-3219.Read more>>
- Hyungtaek Jeonet al. Effects of different separation methods on the physical and functional properties of extracellular vesicles. PLoS One. 2020 Jul 7;15(7):e0235793.Read more>>
- Qinyu Ma. et al. Small extracellular vesicles deliver osteolytic effectors and mediate cancer\induced osteolysis in bone metastatic niche. J Extracell Vesicles. 2021 Feb;10(4):e12068Read more>>
- Mengmeng Liang. et al. Osteoclast-derived small extracellular vesicles induce osteogenic differentiation via inhibiting ARHGAP1. Mol Ther-Nucl Acids. 2021 Mar;23:1191Read more>>
- Lee Jisu. et al. Alpha-2-macroglobulin as a novel diagnostic biomarker for human bladder cancer in urinary extracellular vesicles. FRONT ONCOL. 2022 Sep;0:4727Read more>>
- Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549Read more>>
- Naixin Ding. et al. Mechanism of Exosomal LncRNA PART1 in Esophageal Cancer Angiogenesis by Targeting miR-302a-3p/CDC25A Axis. TECHNOL CANCER RES T. ;()Read more>>
- Li Fu-Xing-Zi. et al. Cold exposure protects against medial arterial calcification development via autophagy. J NANOBIOTECHNOL. 2023 Dec;21(1):1-22Read more>>
- Ting Hong. et al. Exosomal circBBS2 inhibits ferroptosis by targeting miR-494 to activate SLC7A11 signaling in ischemic stroke. FASEB J. 2023 Aug;37(9):e23152Read more>>
- Zheng Ming-Hui. et al. Vascular wall microenvironment: exosomes secreted by adventitial fibroblasts induced vascular calcification. J NANOBIOTECHNOL. 2023 Dec;21(1):1-21Read more>>
- Huang Qun. et al. Bushenhuoluo Decoction improves polycystic ovary syndrome by regulating exosomal miR-30a-5p/ SOCS3/mTOR/NLRP3 signaling-mediated autophagy and pyroptosis. J OVARIAN RES. 2024 Dec;17(1):1-17Read more>>
- Woojin Back. et al. Charge-Based Isolation of Extracellular Vesicles from Human Plasma. ACS OMEGA. 2024;XXXX(XXX):XXX-XXXRead more>>
- Yixuan Liang. et al. Exosomes-mediated transmission of standard bovine viral diarrhea strain OregonC24Va in bovine trophoblast cells. J REPROD IMMUNOL. 2024 Aug;164:104254Read more>>
- Lei Li-Min. et al. Cold exposure-induced plasma exosomes impair bone mass by inhibiting autophagy. J NANOBIOTECHNOL. 2024 Dec;22(1):1-21Read more>>
- Naihan Yuan. et al. CREG1 promotes bovine placental trophoblast cells exosome release by targeting IGF2R and participates in regulating organoid differentiation via exosomes transport. INT J BIOL MACROMOL. 2024 Aug;274:133298Read more>>
VALIDATION IMAGES
L1 rat brain lysates, L2 rat heart lysates probed with Anti-Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted band and observed band size: 42kD
Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-Tsg101 Polyclonal Antibody (bs-1365R), Unconjugated at 1:200 followed by conjugation to the secondary antibody, (SP-0023), and DAB staining
Lane 1: MCF-7 cell lysates probed with Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:500 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Human SH-SY5Y cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HepG2 cell lysates; Lane 4: Human A549 cell lysates probed with Tsg101 Polyclonal Antibody, Unconjugated (bs-1365R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.