bs-1369R
[Primary Antibody]
MAP2/MAP-2a.b.c Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: MAP2/MAP-2a.b.c
Immunogen Range: 1-120/1827
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 4133
Swiss Prot: P11137
Source: KLH conjugated synthetic peptide derived from human MAP2
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 70/201
Cross Reactive Species:
Human
Mouse
Rat
Pig
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Huang, Yabo, et al. "The Expression of FOXJ1 in Neurogenesis after Transient Focal Cerebral Ischemia." The Canadian Journal of Neurological Sciences 40.3 (2013): 403-409.Read more>>
- Zuo, Daiying, et al. "Existence of glia mitigated ketamine-induced neurotoxicity in neuron-glia mixed cultures of neonatal rat cortex and the glia-mediated protective effect of 2-PMPA." Neurotoxicology (2014).Read more>>
- Wang, Jin, et al. "Neuroprotective Effects of Wnt/ß-catenin signaling pathway against Aβ-induced Tau protein over-phosphorylation in PC12 cells."Biochemical and Biophysical Research Communications (2016).Read more>>
- Usui, Tatsuya, et al. "Establishment of a novel three‐dimensional primary culture model for hippocampal neurogenesis." Physiological Reports 5.12 (2017): e13318.Read more>>
- Lu, Lihua, et al. "AMP-activated protein kinase activation in mediating phenylalanine-induced neurotoxicity in experimental models of phenylketonuria." Journal of inherited metabolic disease (2017): 1-9.Read more>>
- Zhou J et al. Imbalance of Microglial TLR4/TREM2 in LPS-Treated APP/PS3 Transgenic Mice: A Potential Link Between Alzheimer's Disease and Systemic Inflammation Neurochemical Research.2019.Read more>>
- Zhang Y et al. Exposure to carbon black nanoparticles during pregnancy persistently damages the cerebrovascular function in female mice. Toxicology. 2019 Apr 22;422:44-52.Read more>>
- Zhang Y et al. Exposure to carbon black nanoparticles during pregnancy persistently damages the cerebrovascular function in female mice. Toxicology. 2019 Apr 22;422:44-52.Read more>>
- Yang YQ et al. Wild-type p53-induced phosphatase 1 down-regulation promotes apoptosis by activating the DNA damage-response pathway in amyotrophic lateral sclerosis. Neurobiol Dis. 2019 Oct 30;134:104648. Read more>>
- Jiang Y et al. A mutation in MAP2 is associated with prenatal hair follicle density. ASEB J. 2019 Dec;33(12):14479-14490. Read more>>
- Che H et al. EPA-Enriched Ethanolamine Plasmalogen and EPA-Enriched Phosphatidylethanolamine Enhance BDNF/TrkB/CREB Signaling and inhibit Neuronal Apoptosis in vitro and in vivo. Food Funct. 2020 Feb 11.Read more>>
- Wang K et al. Harpagide from Scrophularia protects rat cortical neurons from oxygen-glucose deprivation and reoxygenation-induced injury by decreasing endoplasmic reticulum stress. J Ethnopharmacol. 2020 Jan 31;253:112614. Read more>>
- Che H et al. EPA-Enriched Ethanolamine Plasmalogen and EPA-Enriched Phosphatidylethanolamine Enhance BDNF/TrkB/CREB Signaling and inhibit Neuronal Apoptosis in vitro and in vivo. Food Funct. 2020 Feb 11.Read more>>
- Chaohua Cong. et al. PAK4 suppresses motor neuron degeneration in hSOD1G93A\linked amyotrophic lateral sclerosis cell and rat models. 2021 Feb 21Read more>>
- Zhang Heng. et al. Bone Morphogenetic Protein-7 (BMP-7) Promotes Neuronal Differentiation of Bone Marrow Mesenchymal Stem Cells (BMSCs) In Vitro. Biomed Res Int. 2021;2021:7239783Read more>>
- Shanshan Zhang. et al. Pregnancy exposure to carbon black nanoparticles induced neurobehavioral deficits that are associated with altered m6A modification in offspring. Neurotoxicology. 2020 Dec;81:4Read more>>
- Ji-Yue Wen. et al. Endothelium-derived hydrogen sulfide acts as endothelium-derived hyperpolarizing factor and exerts neuroprotective effects via activation of large-conductance Ca2+-activated K+ channels. 2021 Jul 03Read more>>
- Lin Zhu. et al. Neuroprotective effects of salidroside on ageing hippocampal neurons and naturally ageing mice via the PI3K/Akt/TERT pathway. 2021 Aug 09Read more>>
- Yu Hei. et al. Multifunctional Immunoliposomes Enhance the Immunotherapeutic Effects of PD-L1 Antibodies against Melanoma by Reprogramming Immunosuppressive Tumor Microenvironment. 2021 Dec 16Read more>>
- Hongmei Zhou. et al. Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain. Ecotox Environ Safe. 2022 Jan;229:113066Read more>>
- Zhifei Wang. et al. Human Cytomegalovirus Immediate Early Protein 2 Protein Causes Cognitive Disorder by Damaging Synaptic Plasticity in Human Cytomegalovirus-UL122-Tg Mice. Front Aging Neurosci. 2021; 13: 720582Read more>>
- Yan Hu. et al. An ECM-Mimicking, Injectable, Viscoelastic Hydrogel for Treatment of Brain Lesions. ADV HEALTHC MATER. 2022 Nov;:2201594Read more>>
- Lingbing Zhang. et al. Disruption of the lung-gut-brain axis is responsible for cortex damage induced by pulmonary exposure to zinc oxide nanoparticles. TOXICOLOGY. 2022 Dec;:15339Read more>>
- Ma Caiyun. et al. In Vitro Culture and Multipotency Evaluation of Broiler Umbilical Cord Mesenchymal Stem Cells. BRAZ ARCH BIOL TECHN. 2023 Feb;66Read more>>
- Xiahong Tang. et al. Hypoxic preconditioned mesenchymal stem cells ameliorate rat brain injury after cardiopulmonary resuscitation by suppressing neuronal pyroptosis. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE. 2023 MayRead more>>
- Zhan Zhou. et al. Transfer of massive mitochondria from astrocytes reduce propofol neurotoxicity. NEUROSCI LETT. 2024 Jan;818:137542Read more>>
VALIDATION IMAGES
Formalin-fixed and paraffin embedded rat brain labeled with Anti-MAP2/MAP-2A.B.C Polyclonal Antibody, Unconjugated (bs-1369R) followed by conjugation to the secondary antibody and DAPI staining
Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Paraformaldehyde-fixed, paraffin embedded Human brain glioma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with MAP2/MAP-2a.b.c Polyclonal Antibody, Unconjugated (bs-1369R) at 1:400 overnight at 4°C, DAB staining.
SHSY5Y cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with MAP2 Antibody(1369R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).