bs-1371R [Primary Antibody]
SNAI1 Polyclonal Antibody
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DATASHEET

Host: Rabbit

Target Protein: SNAI1

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 6615

Swiss Prot: O95863

Source: KLH conjugated synthetic peptide derived from human Anail

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.

Size: 100µL

Concentration: 1mg/ml

Predicted Molecular Weight: 29


Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Hsu et al. Epidermal growth factor-induced cyclooxygenase-2 enhances head and neck squamous cell carcinoma metastasis through fibronectin up-regulation. (2015) Oncotarge. 6:1723-39Read more>>
  • Jinquan Cai Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma. (2013) Exp.Ther.Me. 6:1489-1493Read more>>
  • Pan et al. MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (2017) Exp.Ther.Med. 14:4663-4670Read more>>
  • Lin et al. Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition. (2016) Front.Pharmacol. 6:320Read more>>
  • Chaudhury et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. (2016) Nat.Commun. 7:13362Read more>>
  • Chaudhury et al. CELF1 is a central node in post-transcriptional regulatory programmes underlying EMT. (2016) Nat.Commun. 7:13362Read more>>
  • Ning et al. Vascular endothelial growth factor receptor-1 activation promotes migration and invasion of breast cancer cells through epithelial-mesenchymal transition. (2013) PLoS.One. 8:e65217Read more>>
  • Li Z et al. Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Exp Eye Res. 2019 Apr 24;184:227-233.Read more>>
  • Li Z et al. Nicotinamide inhibits corneal endothelial mesenchymal transition and accelerates wound healing. Exp Eye Res. 2019 Apr 24;184:227-233.Read more>>
  • Yang Z et al. Tenascin-C is involved in promotion of cancer stemness via the Akt/HIF1ɑ axis in esophageal squamous cell carcinoma.Exp Mol Pathol. 2019 Mar 20.Read more>>
  • Li D et al. Oxygenated Polycyclic aromatic hydrocarbons (Oxy-PAHs) facilitate lung cancer metastasis by epigenetically regulating the epithelial-to-mesenchymal transition (EMT). Environ Pollut. 2019 Sep 17;255(Pt 2):113261. Read more>>
  • Huang T et al. Schwann Cell-Derived CCL2 Promotes the Perineural Invasion of Cervical Cancer. Front Oncol. 2020 Jan 29;10:19.Read more>>
  • Liu H et al. Anti-tubulin agent vinorelbine inhibits metastasis of cancer cells by regulating epithelial-mesenchymal transition. Eur J Med Chem. 2020 Aug 15;200:112332.Read more>>
  • Xin Wang. et al. Multiple roles of FGF10 in the regulation of corneal endothelial wound healing. Exp Eye Res. 2021 Apr;205:108517Read more>>
  • Jun Li. et al. EBV-miR-BART12 inhibits cell migration and proliferation by targeting Snail expression in EBV-associated gastric cancer. 2021 Mar 01Read more>>
  • Zheng Wu. et al. FOXD3 suppresses epithelialCmesenchymal transition through direct transcriptional promotion of SMAD7 in esophageal squamous cell carcinoma. 2021 Sep 22Read more>>
  • Yang Lili. et al. Elucidating the Novel Mechanism of Ligustrazine in Preventing Postoperative Peritoneal Adhesion Formation. Oxid Med Cell Longev. 2022;2022:9226022Read more>>
  • Dr. Jing Tian. et al. Downregulation of SOX21-AS1 alleviated cisplatin resistance in cervical cancer through Epithelial-messenchymal transition (EMT) inhibition. REJUV RES. 2022 Aug 3Read more>>
  • Cheng He. et al. Crosstalk of renal cell carcinoma cells and tumor-associated macrophages aggravates tumor progression by modulating muscleblind-like protein 2/B-cell lymphoma 2/beclin 1-mediated autophagy. CYTOTHERAPY. 2022 OcRead more>>
  • Xin Wenhu. et al. CLCA2 overexpression suppresses epithelial-to-mesenchymal transition in cervical cancer cells through inactivation of ERK/JNK/p38-MAPK signaling pathways. BMC MOL CELL BIOL. 2022 Dec;23(1):1-1Read more>>
  • Xiaomeng Wang. et al. A multifunctional black phosphorus-based adhesive patch intrinsically induces partial EMT for effective burn wound healing. BIOMATER SCI-UK. 2022 NovRead more>>
  • Wenting Liu. et al. ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway. J OCUL PHARMACOL TH. 2022 Dec 21Read more>>
  • Wang Wenjun. et al. LncRNA NEAT1 antagonizes the inhibition of melanoma proliferation, migration, invasion and EMT by Polyphyllin B. N-S ARCH PHARMACOL. 2023 Apr;:1-12Read more>>
  • Yingmin Liu. et al. LOXL2 promotes tumor proliferation and metastasis by FAK/Src signaling in esophageal squamous cell carcinoma. ELECTRON J BIOTECHN. 2023 ApRead more>>
  • Fangyu Chai. et al. Identification of SLC2A3 as a prognostic indicator correlated with the NF-B/EMT axis and immune response in head and neck squamous cell carcinoma. CHANNELS. 2023;17(1):Article: 2208928Read more>>
  • Jinhua Gu. et al. Deciphering the mechanism of Peptostreptococcus anaerobius-induced chemoresistance in colorectal cancer: the important roles of MDSC recruitment and EMT activation. FRONT IMMUNOL. 2023; 14: 1230681Read more>>
  • Jie Chen. et al. Quercetin ameliorates liver fibrosis in Wilson disease and EMT involving suppression of the Hedgehog signaling pathway. ARAB J CHEM. 2024 Jan;17:105487Read more>>
  • Du Xiaoyun. et al. Reduced Proliferative Capacity and Defense against Staphylococcus aureus in Human Nasal Mucosal Epithelium Lacking ZNF365. INT ARCH ALLERGY IMM. 2024 Feb;:1-14Read more>>
  • Tian Jihua. et al. Effects of the S1P/S1PR1 Signaling Pathway on High Glucose-Induced NRK-52E Epithelial-Mesenchymal Transition Via Regulation of ROS/NLRP3. INFLAMMATION. 2024 Aug;:1-15Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human rectal cancer labeled with Anti-Snail/SNAI 1 Polyclonal Antibody, Unconjugated (bs-1371R) followed by conjugation to the secondary antibody and DAB staining


Formalin-fixed and paraffin embedded human pancreas carcinoma labeled with Rabbit Anti-Snail Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 followed by conjugation to the secondary antibody and DAB staining\n


Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining


Paraformaldehyde-fixed, paraffin embedded Rat Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Rat Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


Paraformaldehyde-fixed, paraffin embedded Human Gastric Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SNAI1 Polyclonal Antibody, Unconjugated (bs-1371R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.


25 ug total protein per lane of various lysates (see on figure) probed with SNAI1 polyclonal antibody, unconjugated (bs-1371R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.