bs-13740R-BF647 [Conjugated Primary Antibody]
Smoothelin Polyclonal Antibody, AbBy Fluor-647 Conjugated
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Host: Rabbit

Target Protein: Smoothelin

Clonality: Polyclonal

Isotype: IgG

Source: KLH conjugated synthetic peptide derived from human Smoothelin

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

The cytoskeletal protein smoothelin is highly conserved among vertebrates and is expressed exclusively by contractile smooth muscle cells where it localizes to the filament network. Smoothelin associates with actin stress fibers but does not interact with desmin. At least two isoforms of smoothelin are produced by alternative splicing. The short isoform lacks amino acids 1–544 at the amino-terminus of the long isoform. The short isoform is expressed in visceral muscle tissue, including intestine and stomach, but not in brain, while the long isoform is expressed in all vascularized organs. In the vascular system, smoothelin expression is limited to large veins and arteries capable of pulsatile contraction. As a marker for the highly differentiated contractile smooth muscle cell, smoothelin expression is useful for studying vascular malformation and injury. The gene encoding human smoothelin maps to chromosome 22q12.2.

Conjugation: AbBy Fluor-647

Excitation/ Emission: 650nm/665nm

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Prayitnaningsih et al. Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats. (2016) Int.J.Ophthalmol. 9:1568-1577Read more>>
  • Han X et al. The intervention effect of nicotine on cervical fibroblast-myofibroblast differentiation in lipopolysaccharide-induced preterm birth model through activating the TGF-1/Smad3 pathwayBiomed Pharmacother.2020 Dec 24;134:111135.Read more>>
  • Guoyong Zhang. et al. Buyang Huanwu Decoction suppresses cardiac inflammation and fibrosis in mice after myocardial infarction through inhibition of the TLR4 signalling pathway. J ETHNOPHARMACOL. 2023 Nov;:117388Read more>>
VALIDATION IMAGES