bs-1380R
[Primary Antibody]
GPR30 Polyclonal Antibody
DATASHEET
Host: Rabbit
Target Protein: GPR30
Immunogen Range: 251-375/375
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 2852
Swiss Prot: Q99527
Source: KLH conjugated synthetic peptide derived from human GPR30
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK. Mediates pleiotropic functions among others in the cardiovascular, endocrine, reproductive, immune and central nervous systems. Has a role in cardioprotection by reducing cardiac hypertrophy and perivascular fibrosis in a RAMP3-dependent manner. Regulates arterial blood pressure by stimulating vasodilation and reducing vascular smooth muscle and microvascular endothelial cell proliferation. Plays a role in blood glucose homeostasis contributing to the insulin secretion response by pancreatic beta cells. Triggers mitochondrial apoptosis during pachytene spermatocyte differentiation. Stimulates uterine epithelial cell proliferation. Enhances uterine contractility in response to oxytocin. Contributes to thymic atrophy by inducing apoptosis. Attenuates TNF-mediated endothelial expression of leukocyte adhesion molecules. Promotes neuritogenesis in developing hippocampal neurons. Plays a role in acute neuroprotection against NMDA-induced excitotoxic neuronal death. Increases firing activity and intracellular calcium oscillations in luteinizing hormone-releasing hormone (LHRH) neurons. Inhibits early osteoblast proliferation at growth plate during skeletal development. Inhibits mature adipocyte differentiation and lipid accumulation. Involved in the recruitment of beta-arrestin 2 ARRB2 at the plasma membrane in epithelial cells.
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
FCM(1:20-100)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 42
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Cow
Horse
Chicken
Rabbit
For research use only. Not intended for diagnostic or therapeutic use.
PRODUCT SPECIFIC PUBLICATIONS
- Zhao, Zhuo, et al. "GPR30 Decreases Cardiac Chymase/Angiotensin II by Inhibiting Local Mast Cell Number." Biochemical and Biophysical Research Communications (2015).Read more>>
- Wang, Hao, et al. "Activation of GPR30 inhibits cardiac fibroblast proliferation."Molecular and Cellular Biochemistry: 1-14.Read more>>
- Wang, Hao, et al. "Activation of GPR30 improves exercise capacity and skeletal muscle strength in senescent female Fischer344× Brown Norway rats." Biochemical and Biophysical Research Communications (2016).Read more>>
- Wenbo Ge. et al. 17-estradiol protects sheep oviduct epithelial cells against lipopolysaccharide-induced inflammation in vitro. Mol Immunol. 2020 Nov;127:21Read more>>
- Andressa S. Sousa. et al. Evaluation of coronary function in female rats with severe type 1 diabetes: Effects of combined treatment with insulin and pyridoxamine. MICROVASC RES. 2022 Dec;:104474Read more>>
- Ting Chen. et al. Tongmai Yangxin pill alleviates myocardial no-reflow by activating GPER to regulate HIF-1 signaling and downstream potassium channels. PHARM BIOL. 2023;61(1):499-513Read more>>
- Qianfeng Liu. et al. Perfluoroalkyl substances promote breast cancer progression via ER and GPER mediated PI3K/Akt and MAPK/Erk signaling pathways. ECOTOX ENVIRON SAFE. 2023 Jun;258:11498Read more>>
- Murakami Hiromoto. et al. Equol, a soybean metabolite with estrogen-like functions, decreases lipopolysaccharide-induced human neutrophil extracellular traps in vitro. SHOCK. 2023 Nov;:10.1097/SHK.0000000000002273Read more>>
VALIDATION IMAGES
Mouse brain lysates probed with GPR30 Polyclonal Antibody, unconjugated (bs-1380R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse heart lysates probed with GPR30 Polyclonal Antibody, unconjugated (bs-1380R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
L1 human colon carcinoma, L2 mouse brain lysates probed (bs-1380R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in RT. Predicted and observed band size: 42kDa.\n
Formalin-fixed and paraffin embedded human gastric adenocarcinoma labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:300 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human rectal carcinoma tissue labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human rectal carcinoma tissue labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Formalin-fixed and paraffin embedded human rectal carcinoma tissue labeled with Rabbit Anti-GPR30 Polyclonal Antibody (bs-1380R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
A431 cells probed with GPR30 Polyclonal Antibody, unconjugated (bs-1380R) at 1:100 dilution for 30 minutes compared to control cells (dark blue), secondary only (light blue) and isotype control (orange)
Paraformaldehyde-fixed, paraffin embedded Rat uterus; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with GPR30 Polyclonal Antibody, Unconjugated (bs-1380R) at 1:200 overnight at 4°C, DAB staining.
Tissue/cell:sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GPR30) polyclonal Antibody, Unconjugated (bs-1380R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Tissue/cell:sh-sy5y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (GPR30) polyclonal Antibody, Unconjugated (bs-1380R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.