bs-1384R [Primary Antibody]
ATG4B Polyclonal Antibody
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]

Host: Rabbit

Target Protein: ATG4B

Immunogen Range: 85-200/392

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 23192

Swiss Prot: Q9Y4P1

Source: KLH conjugated synthetic peptide derived from human ATG4B

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.


Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Size: 100ul

Concentration: 1ug/ul

Applications: ELISA(1:500-1000)

Predicted Molecular Weight: 44

Cross Reactive Species: Human

Predicted Cross Reactive Species: Dog

For research use only. Not intended for diagnostic or therapeutic use.


Image kindly submitted by M. G. Connor as part of the free sample program. The bacteria Zprojection image figure legend: RAW264.7 Macrophage cells infected with GFP-bacteria (MOI3), stained with ATG4B primary antibody (1:1000) and visualized with Alexa Flour 595 (red). Nucleus is stained with DAPI (blue). Coverslips mounted with Prolong Gold Antifade with DAPI. Zprojections generated using FIJI. Scale bar is 5um. Images courtesy of M. G. Connor 2014

Formalin-fixed and paraffin embedded mouse liver labeled with Anti-APG4B/AUTL1 Polyclonal Antibody, Unconjugated (bs-1384R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.