bs-1389R [Primary Antibody]
Cyclin G Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: Cyclin G

Immunogen Range: 181-295/295


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 900

Swiss Prot: P51959

Source: KLH conjugated synthetic peptide derived from human Cyclin G

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

May play a role in growth regulation. Is associated with G2/M phase arrest in response to DNA damage. May be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation (By similarity).

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)

Predicted Molecular Weight: 34


Cross Reactive Species: Human
Mouse

Predicted Cross Reactive Species: Rat
Dog
Cow
Pig
Horse
Chicken
Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Xu, Qian, et al. "CYCLIN G1 INHIBITS THE PROLIFERATION OF MOUSE ENDOMETRIAL STROMAL CELL IN DECIDUALIZATION."Read more>>
VALIDATION IMAGES

Mouse liver lysates probed with Rabbit Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.


Mouse kidney lysates probed with Rabbit Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.


Mouse spleen lysates probed with Rabbit Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody for 90 min at 37˚C.


Formalin-fixed and paraffin embedded human endometrium carcinoma tissue labeled with Anti-Cyclin G Polyclonal Antibody, Unconjugated (bs-1389R) at 1:200 followed by conjugation to the secondary antibody and DAB stainin


HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Cyclin G) polyclonal Antibody, Unconjugated (bs-1389R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.