bs-13976R-FITC

DATASHEET

Host: Rabbit

Target Protein: COP1 (Ser387)

Modification Site: Ser387

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 64326

Swiss Prot: Q8NHY2

Source: KLH conjugated synthesised phosphopeptide derived from human COP1 around the phosphorylation site of Ser387

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Background:

COP1 is an E3 ubiquitin ligase protein that mediates ubiquitination and degradation of target proteins such as c-Jun and p53. It is a component of the DCX DET1-COP1 ubiquitin ligase complex which consists of RBX1, DET1, DDB1, CUL4A and COP1. Localizing to the cytoplasm and to the nucleus, COP1 is primarily expressed in testis, placenta, heart and skeletal muscle. COP1 is a potent inhibitor of p53-dependent transcription and apoptosis but, when phosphorylated by Atm (ataxia telangiectasia mutated) in response to DNA damage, the COP1-p53 complex is disrupted and p53 is allowed to exert its pro-apoptotic properties. In ovarian and breast cancers, COP1 is overexpressed, suggesting a role for COP1 in tumorigenesis.

Conjugation: FITC

Excitation/ Emission: 494nm/518nm

Size: 100µL

Concentration: 1ug/ul

Applications: IF(ICC)(1:50-200)
IF()

Predicted Molecular Weight: 80 kDa

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.