bs-13987R [Primary Antibody]
COQ6 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: COQ6

Immunogen Range: 51-150/468


Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 51004

Swiss Prot: Q9Y2Z9

Source: KLH conjugated synthetic peptide derived from human COQ6

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Background:

FAD-dependent monooxygenase required for the C5-ring hydroxylation during ubiquinone biosynthesis. Catalyzes the hydroxylation of 3-polyprenyl-4-hydroxybenzoic acid to 3-polyprenyl-4,5-dihydroxybenzoic acid. The electrons required for the hydroxylation reaction may be funneled indirectly from NADPH via a ferredoxin/ferredoxin reductase system to COQ6.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
ICC(1:100-500)

Predicted Molecular Weight: 51


Cross Reactive Species: Human
Mouse
Rat

Predicted Cross Reactive Species: Rabbit

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation withCOQ6 Polyclonal Antibody, Unconjugated (bs-13987R) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining.


Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-COQ6 Polyclonal Antibody, Unconjugated (bs-13987R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.


Formalin-fixed and paraffin embedded mouse brain labeled with Anti-COQ6 Polyclonal Antibody, Unconjugated (bs-13987R) at 1:500 followed by conjugation to the secondary antibody and DAB staining.


A549 lysates probed with COQ6 Polyclonal Antibody, Unconjugated (bs-13987R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.