bs-1399R [Primary Antibody]
E2F4 Polyclonal Antibody
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Host: Rabbit

Target Protein: E2F4

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 1874

Source: KLH conjugated synthetic peptide derived from human E2F4

Purification: Purified by Protein A.

Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

Storage: Store at -20°C for 12 months.

Background:

E2Fs are DNA binding proteins, which associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F4 is regulated by complex formation with Rb p110. E2F family members bind DNA as heterodimers with members of the DP family of polypeptides.

Size: 100µL

Concentration: 1ug/ul

Applications: WB(1:300-5000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

PRODUCT SPECIFIC PUBLICATIONS
  • Zou, Xiaopan. et al. ZMIZ2 promotes the development of triple-receptor negative breast cancer. Cancer Cell Int. 2022 Dec;22(1):1-16Read more>>
VALIDATION IMAGES

Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-E2F4 Polyclonal Antibody, Unconjugated (bs-1399R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Lane 1: Human A431 cell lysates; Lane 2: Human 293T cell lysates; Lane 3: Human HL-60 cell lysates probed with E2F4 Polyclonal Antibody, Unconjugated (bs-1399R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at room temperature for 60 min.