Host: Rabbit
Target Protein: SRF
Immunogen Range: 101-250/508
Clonality: Polyclonal
Isotype: IgG
Entrez Gene: 6722
Swiss Prot: P11831
Source: KLH conjugated synthetic peptide derived from human SRF
Purification: Purified by Protein A.
Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Background:
Size: 100ul
Concentration: 1ug/ul
Applications:
WB(1:300-5000)
ELISA(1:500-1000)
IHC-P(1:200-400)
IHC-F(1:100-500)
IF(IHC-P)(1:50-200)
IF(IHC-F)(1:50-200)
IF(ICC)(1:50-200)
Predicted Molecular Weight: 56
Cross Reactive Species:
Human
Mouse
Rat
Predicted Cross Reactive Species:
Dog
Cow
Pig
For research use only. Not intended for diagnostic or therapeutic use.
Mouse large intestine lysates probed with Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse muscle lysates probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Mouse NIH/3T3 cells probed with SRF Polyclonal Antibody, unconjugated (bs-1401R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.
Formalin-fixed and paraffin embedded rat brain labeled with Anti-SRF/SAP2/MCM1 Polyclonal Antibody, Unconjugated (bs-1401R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
Lane 1: A431 cell lysates; Lane 2: Hela cell lysates; Lane 3: 293T cell lysates probed with SRF Polyclonal Antibody, Unconjugated (bs-1401R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C