bs-1417R [Primary Antibody]
NFAT2 Polyclonal Antibody
www.biossusa.com
[email protected]
800.501.7654 [DOMESTIC]
+1.781.569.5821 [INTERNATIONAL]
DATASHEET

Host: Rabbit

Target Protein: NFAT2

Immunogen Range: 660-710/716

Clonality: Polyclonal

Isotype: IgG

Entrez Gene: 4772

Swiss Prot: O95644

Source: KLH conjugated synthetic peptide derived from human NFATc1

Purification: Purified by Protein A.

Storage Buffer: Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide.

Storage Condition: Store at -20°C for 12 months.

Background: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells.

Size: 100ul

Concentration: 1ug/ul

Applications: WB(1:300-1000)
IHC-P(1:200-400)
IF(IHC-P)(1:50-200)

Cross Reactive Species: Human
Mouse
Rat

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Formalin-fixed and paraffin embedded mouse lymphoma labeled with Anti-NFATC1/NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.


Mouse Hippocampal tissue labeled with Anti-NFATc1/NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) followed by conjugation to the secondary antibody


Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with NFAT2 Polyclonal Antibody, Unconjugated (bs-1417R) at 1:400 overnight at 4°C, DAB staining.